A new fluorescent glucose assay has been created using Alexa Fluor 647-labeled concanavalin A (Con A) and a fourth-generation PAMAM Alexa Fluor 594-labeled glycodendrimer. This assay has been shown to have a large response to glucose within the biological range and to be capable of functioning within a polymer hydrogel. ... More
The plane of first cleavage is not related to the distribution of sperm components in the mouse.
AuthorsDavies TJ, Gardner RL
JournalHum Reprod
PubMed ID12202427
BACKGROUND: Marking experiments using phytohaemagglutinin (PHA) and Concanavalin A (ConA) have suggested that the first cleavage plane is related to the point of sperm entry. Because of concerns about the specificity of lectin binding, the distribution of sperm components has been investigated directly. METHODS AND RESULTS: The sperm tail could ... More
Elimination of plasma membrane phosphatidylinositol (4,5)-bisphosphate is required for exocytosis from mast cells.
AuthorsHammond GR, Dove SK, Nicol A, Pinxteren JA, Zicha D, Schiavo G
JournalJ Cell Sci
PubMed ID16687737
The inositol lipid phosphatidylinositol (4,5)-bisphosphate [PtdIns(4,5)P2] is involved in a myriad of cellular processes, including the regulation of exocytosis and endocytosis. In this paper, we address the role of PtdIns(4,5)P2 in compound exocytosis from rat peritoneal mast cells. This process involves granule-plasma membrane fusion as well as homotypic granule membrane ... More
Acquisition of Phage Sensitivity by Bacteria through Exchange of Phage Receptors.
AuthorsTzipilevich E, Habusha M, Ben-Yehuda S
JournalCell
PubMed ID28041851
'Bacteriophages (phages) typically exhibit a narrow host range, yet they tremendously impact horizontal gene transfer (HGT). Here, we investigate phage dynamics in communities harboring phage-resistant (R) and sensitive (S) bacteria, a common scenario in nature. Using Bacillus subtilis and its lytic phage SPP1, we demonstrate that R cells, lacking SPP1 ... More
Mutations in EEA1 are associated with allergic bronchopulmonary aspergillosis and affect phagocytosis of Aspergillus fumigatus by human macrophages.
AuthorsOverton NLD, Brakhage AA, Thywißen A, Denning DW, Bowyer P
JournalPLoS One
PubMed ID29547649
'Allergic bronchopulmonary aspergillosis (ABPA) in asthma is a severe, life-affecting disease that potentially affects over 4.8 million people globally. In the UK, ABPA is predominantly caused by the fungus Aspergillus fumigatus. Phagocytosis is important in clearance of this fungus, and Early Endosome Antigen 1 (EEA1) has been demonstrated to be ... More
Reactive oxygen species drive evolution of pro-biofilm variants in pathogens by modulating cyclic-di-GMP levels.
Authors
JournalOpen Biol
PubMed ID27881736
Surface Expression of Kynurenine 3-Monooxygenase Promotes Proliferation and Metastasis in Triple-Negative Breast Cancers.
Authors
JournalCancer Control
PubMed ID33887987
Selected Amino Acids Promote Mouse Pre-implantation Embryo Development in a Growth Factor-Like Manner.
Authors
JournalFront Physiol
PubMed ID32210831
Live Imaging of a Hyperthermophilic Archaeon Reveals Distinct Roles for Two ESCRT-III Homologs in Ensuring a Robust and Symmetric Division.
Authors
JournalCurr Biol
PubMed ID32502411
Employing high-frequency alternating magnetic fields for the non-invasive treatment of prosthetic joint infections.
Authors
JournalSci Rep
PubMed ID28790407
Quantitation of yeast cell-cell fusion using multicolor flow cytometry.
Authors
JournalCytometry A
PubMed ID26033928
Evidence of the presence of nucleic acids and ß-glucan in the matrix of non-typeable Haemophilus influenzae in vitro biofilms.
AuthorsDomenech M, Pedrero-Vega E, Prieto A, García E
JournalSci Rep
PubMed ID27805043
Non-typeable Haemophilus influenzae (NTHi) is a Gram-negative bacterium that frequently colonizes the human nasopharynx; it is a common cause of chronic and recurrent otitis media in children and of exacerbations of chronic obstructive pulmonary disease. To date, no exopolysaccharide clearly contributing to NTHi biofilms has been identified. Consequently, there is ... More
Increased intracellular pH is necessary for adult epithelial and embryonic stem cell differentiation.
Despite extensive knowledge about the transcriptional regulation of stem cell differentiation, less is known about the role of dynamic cytosolic cues. We report that an increase in intracellular pH (pHi) is necessary for the efficient differentiation of Drosophila adult follicle stem cells (FSCs) and mouse embryonic stem cells (mESCs). We ... More
A simple and robust fluorescent labeling method to quantify trophoblast fusion.
AuthorsZhang Y, Yang H
JournalPlacenta
PubMed ID30827351
Trophoblast fusion into syncytiotrophoblasts is a specialized yet enigmatic cellular process, which is essential for placental development and function. To facilitate mechanistic understanding of this critical process, here we re-purposed a widely used fluorescent membrane potential dye, Di-8-ANEPPS, to stably label the plasma membrane of live BeWo trophoblast cells. Compared ... More