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Citas y referencias (215)
Invitrogen™
5-FAM, SE (5-Carboxyfluorescein, Succinimidyl Ester), single isomer
¿Busca alternativas mejores a la fluoresceína? Nuestra serie de colorantes Alexa Fluor le ofrece todo lo que busca y muchoMás información
| Número de catálogo | Cantidad |
|---|---|
| C2210 también denominado C-2210 | 10 mg |
Número de catálogo C2210
también denominado C-2210
Precio (MXN)
-
Cantidad:
10 mg
¿Busca alternativas mejores a la fluoresceína? Nuestra serie de colorantes Alexa Fluor le ofrece todo lo que busca y mucho más.
Para uso exclusivo en investigación. No apto para uso en procedimientos diagnósticos.
Especificaciones
Reactividad químicaAmina
Etiqueta o tinteFITC (fluoresceína)
Tipo de producto5-FAM SE
Cantidad10 mg
Fracción reactivaEster activo, succinimidilo éster
Condiciones de envíoTemperatura ambiente
Tipo de etiquetaColorantes clásicos
Unit SizeEach
Contenido y almacenamiento
Almacenar en el congelador (de – 5 a – 30 °C) y proteger de la luz.
Citations & References (215)
Citations & References
Abstract
Flow cytometric analysis of the in situ accessibility of Escherichia coli 16S rRNA for fluorescently labeled oligonucleotide probes.
Journal:Applied and environmental microbiology
PubMed ID:9835591
In situ identification of whole fixed bacterial cells by hybridization with fluorescently labeled, rRNA-targeted oligonucleotide probes is often limited by low signal intensities. In addition to an impermeability of the cell periphery and a low cellular rRNA content, the three-dimensional structure of the ribosome may hinder the access of oligonucleotides
Mechanism of peptide-induced mast cell degranulation. Translocation and patch-clamp studies.
Journal:J Gen Physiol
PubMed ID:9806967
Substance P and other polycationic peptides are thought to stimulate mast cell degranulation via direct activation of G proteins. We investigated the ability of extracellularly applied substance P to translocate into mast cells and the ability of intracellularly applied substance P to stimulate degranulation. In addition, we studied by reverse
Quantitative polymerase chain reaction-based homogeneous assay with fluorogenic probes to measure c-erbB-2 oncogene amplification.
Journal:Clin Chem
PubMed ID:9166227
We describe a PCR-based assay for determining c-erbB-2 oncogene amplification in breast cancer in which we use the TaqMan system. Two fluorogenic probes anneal to the target between primers for c-erbB-2 and beta-globin genes and contain both a reporter dye (6-carboxy-fluorescein) and a quencher dye (6-carboxy-tetramethyl-rhodamine). During the extension phase
DNA sequencing with dye-labeled terminators and T7 DNA polymerase: effect of dyes and dNTPs on incorporation of dye-terminators and probability analysis of termination fragments.
Journal:Nucleic Acids Res
PubMed ID:1598205
The incorporation of fluorescently labeled dideoxynucleotides by T7 DNA polymerase is optimized by the use of Mn2+, fluorescein analogs and four 2'-deoxyribonucleoside 5'-O-(1-thiotriphosphates) (dNTP alpha S's). The one-tube extension protocol was tested on single-stranded templates, as well as PCR fragments which were made single-stranded by digestion with T7 gene 6
Fluorescence polarization immunoassay IV. Determination of phenytoin and phenobarbital in human serum and plasma.
Journal:Clin Chem
PubMed ID:6751601
Fluorescence polarization immunoassays for phenytoin and phenobarbital in human serum or plasma are described and shown to be clinically useful. No sample pretreatment, extraction, or phase separation is required. A determination can be made with less than 20 microL of sample in 15 min, with incubation at ambient temperature. Abnormal