Invitrogen™

Calcium Green™-1, AM, cell permeant

Catalog number: C3012
Invitrogen™

Calcium Green™-1, AM, cell permeant

Catalog number: C3012
Catalog Number
C3012
also known as C-3012
Unit Size
10 x 50 µg
Price (USD)
387.00
Your Price:
Availability
***
Quantity
Catalog Number
C3011MP
also known as C-3011MP
Unit Size
500 μg
Price (USD)
318.00
Your Price:
Availability
***
Quantity
Catalog NumberUnit SizePrice (USD)AvailabilityQuantity
C3012
also known as C-3012
10 x 50 µg
387.00
Your Price:
***
C3011MP
also known as C-3011MP
500 μg
318.00
Your Price:
***
Product Overview
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Additional Information
Recommendations
Recommendations
Labeled calcium indicators are molecules that exhibit an increase in fluorescence upon binding Ca2+. They have uses in many calcium signaling investigations, including measuring intracellular Ca2+, following Ca2+ influx and release, and multiphoton excitation imaging of Ca2+ in living tissues. Cells may be loaded with the AM ester forms of these calcium indicators by adding the dissolved indicator directly to dishes containing cultured cells. The fluorescence signal from these cells is generally measured using fluorescence microscopy, fluorescence microplate assays, or flow cytometry.

Calcium Indicator (AM Ester) Specifications:
• Label (Ex/Em): Calcium Green™ -1 (506/531 nm)
• Fluorescence intensity increase upon binding Ca2+ : ∼14 fold
• Exhibit fluorescence increase upon binding Ca2+ with little shift in wavelength


Spectral Characteristics of Molecular Probes™ Calcium Indicators
These probes are excited by visible light, and because the energy required for excitation is low, the potential for cellular photodamage is reduced. Commonly used laser-based instruments (i.e., confocal laser scanning microscopes) are able to efficiently excite these indicators, and their emissions are in regions of the spectrum where cellular autofluorescence and scattering backgrounds are often less of a problem.

More Choices for Fluorescent Calcium Indicators
We offer a large selection of Molecular Probes™ calcium indicators for use in various experimental scenarios, for example dextran versions for reduced leakage and compartmentalization and BAPTA conjugates for detecting high-amplitude calcium transients. For more information, review Fluorescent Ca2+ Indicators Excited with Visible Light—Section 19.3 in the Molecular Probes™ Handbook.

For UV-excitable Ca2+ indicators, protein-based Ca2+ indicators, conjugates of Ca2+ indicators, and for fluorescence-based indicators of other metal ions (i.e., Mg2+, Zn2+) review Indicators for Ca2+, Mg2+, Zn2+ and Other Metal Ions—Chapter 19 in the Molecular Probes™ Handbook.

For Research Use Only. Not intended for animal or human therapeutic or diagnostic use.

Specifications

Detection Method
Fluorescent
For Use With (Application)
Cell Viability & Proliferation
For Use With (Equipment)
Fluorescence Microscope
Product Type
Calcium Indicator
Dye Type
Fluorescent Dye-Based
Product Line
Calcium Green™
Quantity
10 x 50μg
Shipping Condition
Room Temperature

Contents & Storage

Store in freezer -5°C to -30°C and protect from light.

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