Invitrogen™

One Shot™ TOP10F´ Chemically Competent E. coli

Catalog number: C303003
Invitrogen™

One Shot™ TOP10F´ Chemically Competent E. coli

Catalog number: C303003
Catalog Number
C303003
Unit Size
21 x 50 µL/tube
Price (USD)
Price: 453.00
Your Price:
Availability
-
Quantity
Catalog Number
Unit Size
42 x 50 µL/tube
Price (USD)
Price: 852.00
Your Price:
Availability
-
Quantity
Catalog NumberUnit SizePrice (USD)AvailabilityQuantity
C30300321 x 50 µL/tube
Price: 453.00
Your Price:
-
C30300642 x 50 µL/tube
Price: 852.00
Your Price:
-
Product Overview
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Additional Information
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One Shot™ TOP10F´ Chemically Competent E. coli are identical to TOP10 cells, with the addition of an F´ episome. TOP10 competent cells are provided at a transformation efficiency of 1 x 109 cfu/µg supercoiled DNA and are ideal for high-efficiency cloning and plasmid propagation.

Using One Shot™ TOP10F´ Chemically Competent E. coli
The F´ episome carries the tetracycline resistance gene and allows isolation of single-stranded DNA from vectors that have an f1 origin of replication. In addition, the F´ episome carries the lacIq repressor for inducible expression from trc, tac, and lac promoters using IPTG. TOP10F´ cells require IPTG induction for blue/white color screening.
For Research Use Only. Not for use in diagnostic procedures.

Specifications

Product Type
Competent Cell
Contains F' Episome
Contains F' Episome
Efficiency
High Efficiency (>10^9 cfu/µg)
Cell Type
Chemically Competent
Improves Plasmid Quality
Yes
Cloning Methylated DNA
Yes
Transformation Efficiency Level
High Efficiency (> 10^9 cfu⁄µg)
Cloning Unstable DNA
Not Suitable for cloning unstable DNA
Bacterial or Yeast Strain
TOP10F´
Blue/White Screening
Yes
High-throughput Compatibility
Not High-throughput Compatible (Manual)
Preparing Unmethylated DNA
Not Suitable for preparing unmethylated DNA
Propagating ccdB Vectors
Not for ccdB vector propagation
Reduces Recombination
Yes
T1 Phage - Resistant (tonA)
No
Species
E. coli
Promoter
Trc, Tac, Lac
Format
One Shot
Product Line
One Shot™
Quantity
20 x 50 μL

Contents & Storage

Contains:
• One Shot™ TOP10F′ Chemically Competent E. coli: 21 vials, 50 µl each
• pUC19 DNA (10 pg/µl): 1 vial, 50 µl
• S.O.C. medium: 1 bottle, 6 ml

Store Competent Cells at -80°C. Store pUC19 DNA at -20°C. Store SOC Medium at 4°C or at room temperature.