Invitrogen™

One Shot™ TOP10F´ Chemically Competent E. coli

Catalog number: C303006
Invitrogen™

One Shot™ TOP10F´ Chemically Competent E. coli

Catalog number: C303006
Catalog Number
C303006
also known as C3030-06
Unit Size
42 x 50 µL/tube
Price (USD)
Availability
-
Quantity
Catalog Number
Unit Size
21 x 50 µL/tube
Price (USD)
Availability
-
Quantity
Catalog NumberUnit SizePrice (USD)AvailabilityQuantity
C303006
also known as C3030-06
42 x 50 µL/tube
-
C30300321 x 50 µL/tube
-
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One Shot TOP10F´ Chemically Competent E. coli are identical to TOP10 cells, with the addition of an F´ episome. The F´ episome carries a tetracycline resistance gene and allows isolation of single-stranded DNA (ssDNA) from vectors that have an f1 origin of replication. In addition, the F´ episome carries the lacIq repressor for inducible expression from trc, tac, and lac promoters using IPTG.

TOP10F´ competent cells are provided at a transformation efficiency of >1 x 109 cfu/μg supercoiled DNA and are ideal for high-efficiency cloning and plasmid propagation.

As a derivative of TOP10, TOP10F´ Chemically Competent E. coli cells carry mutations in the methylation-dependent restriction system (mcrA, mcrBC, and mrr) allowing them to clone both prokaryotic and eukaryotic genomic DNA and provide efficient plasmid rescue from eukaryotic genomes. TOP10F´ cells also carry recA1 and endA1 mutations that increase insert stability and improve the quality of plasmid DNA prepared from minipreps.

One Shot TOP10F´ Chemically Competent E. coli cells offer:
• Transformation efficiencies of >1 x 109 cfu/μg
• Stable F´ episome that allows isolation of ssDNA and carries lacIq repressor for inducible expression from trc, tac, and lac promoters
hsdR for efficient transformation of unmethylated DNA from PCR amplifications
mcrA for efficient transformation of methylated DNA from genomic preparations
lacZΔM15 for blue/white color screening of recombinant clones on plates containing X-Gal or Bluo-Gal
endA1 for cleaner DNA preparations and better results in downstream applications due to elimination of nonspecific digestion by Endonuclease I
recA1 for reduced occurrence of nonspecific recombination in cloned DNA

Note: IPTG induction is required for blue-white color screening.

Easy-to-use One Shot format
TOP10 F´ Chemically Competent E. coli cells are supplied in the convenient, single-reaction One Shot format. The single-tube, single-use format allows all steps of the transformation protocol, up to plating, to take place in the same tube, saving time and preventing contamination.

Genotype
F´{lacIq, Tn10(TetR)} mcrA Δ(mrr-hsdRMS-mcrBC) φ80lacZΔM15 ΔlacX74 recA1 araD139 Δ(ara-leu)7697 galU galK rpsL(StrR) endA1 nupG

Find the strains and formats that fit your needs
DH strains are available in chemically competent and electrocompetent cell formats.
For high throughput applications there are a variety of TOP10 strains in MultiShot formats.
For ssDNA production try strains such as TOP10F´ or electrocompetent DH12S.
Explore bacterial growth media formats.

For Research Use Only. Not for use in diagnostic procedures.

Specifications

Product Type
Chemically Competent Cells
Contains F' Episome
Yes
Improves Plasmid Quality
Yes (endA1)
Cloning Methylated DNA
Yes (mcrA)
Transformation Efficiency Level
High Efficiency (>1 x 109 cfu/μg)
Antibiotic Resistance Bacterial
Yes (Streptamycin, Tetracycline)
Cloning Unstable DNA
Not suitable for cloning unstable DNA
Blue/White Screening
Yes (lacZΔM15)
High-throughput Compatibility
Low
Preparing Unmethylated DNA
No
Reduces Recombination
Yes (recA1)
Shipping Condition
Dry Ice
T1 Phage - Resistant (tonA)
No
Species
E. coli (K12)
Promoter
Trc, Tac, Lac
Format
Tube
Product Line
One Shot™
Quantity
42 x 50 μL

Contents & Storage

• One Shot TOP10F' Chemically Competent E. coli (42 x 50 μL)
Store Competent Cells at –80°C.

• pUC19 plasmid (50 μL at 10 pg/μL)
Store pUC19 plasmid at –20°C.

• S.O.C. Medium (6 mL)
Store S.O.C. Medium at 4°C or room temperature.

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