Calceína, AM, colorante de color persignificado celular
Calceína, AM, colorante de color persignificado celular
Citas y referencias (658)
Invitrogen™

Calceína, AM, colorante de color persignificado celular

La calceína AM es un colorante que penetra en la célula y que puede utilizarse para determinar la viabilidad celularMás información
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Número de catálogoTipo de productoCantidadColor
C3099Calcein, AM1 mLGreen
C3100MPCalcein, AM20 x 50 μgGreen
C1430Calcein, AM1 mgGreen
C1429AM de azul de la calceína1 mgBlue
C34852Calceína AM de color verde; embalaje especial20 x 50 μgGreen
C481Calceína100 mgGreen
Número de catálogo C3099
Precio (MXN)
-
Tipo de producto:
Calcein, AM
Cantidad:
1 mL
Color:
Green
La calceína AM es un colorante que penetra en la célula y que puede utilizarse para determinar la viabilidad celular en la mayoría de las células eucariotas. En células vivas, la calceína AM no fluorescente se convierte en calceína con fluorescencia de verde tras la hidrólisis del acetoximetil éster por parte de las esterasas intracelulares. Este colorante también está disponible como 1 mg del sólido (C-1430) y resuspendido en dimetilsulfóxido (DMSO) (C-3099). Para una versión de longitud de onda más larga de este colorante, ofrecemos la nueva calceína AM de color rojo-anaranjado CellTrace (C-34851).
Para uso exclusivo en investigación. No apto para uso en procedimientos diagnósticos.
Especificaciones
Permeabilidad celularPermeabilidad celular
Concentración1 mg⁄ml
Tipo de coloranteOtras etiquetas o colorantes
FormularioSolution in DMSO
Cantidad1 mL
Tipo de reactivoCompuestos de seguimiento celular, reactivos de etiquetado celular
Condiciones de envíoTemperatura ambiente
Enzima dianaEsterasa
ColorGreen
Emission517 nm
Excitation Wavelength Range494 nm
Para utilizar con (aplicación)Rastreo celular, rastreador celular, Cell Tracker
Para utilizar con (equipo)Microscopio de fluorescencia
Tipo de productoCalcein, AM
Unit SizeEach
Contenido y almacenamiento
Almacenar en el congelador de -5 °C a -30 °C y proteger de la luz.

Preguntas frecuentes

I would like a dye to load in live cells such that it will self-quench at a high concentration, but if the cell dies, the dye will be released and unquenched. Do you have anything like that?

Yes. This is commonly done with calcein AM or FDA (fluorescein diacetate). These dyes will not fluoresce until cleaved by esterases. After modification by esterases and at very high concentrations, they will self-quench. Upon disruption of the plasma membrane, or cell death, the dye will be released into the extracellular medium, and become unquenched. Concentration and incubation time must be optimized to obtain adequate quenching.

Find additional tips, troubleshooting help, and resources within our Cell Analysis Support Center.

I need to load liposomes with calcein. Should I use the AM form or the non-AM form?

Calcein, AM requires esterase cleavage of the acetoxymethyl (AM) ester to become fluorescent. Liposomes don't have esterases unless specifically constructed to include the enzyme. The water-soluble, non-AM form of calcein (Cat. No. C481), does not require esterase cleavage to be fluorescent.

Find additional tips, troubleshooting help, and resources within our Cell Analysis Support Center.

I want to do a cell migration study for around 4 hours and need to fluorescently label the cells with a dye. What do you recommend?

Calcein, AM and FDA (fluorescein diaceate) are examples of some dyes used for this application. Since these dyes are not incorporated or covalently attached to any cellular components, they may have a short retention time as some cell types may actively efflux the dye out of the cells. The CellTracker and CellTrace dyes include either a mild thiol-reactive chloromethyl group or amine-reactive succinnimidyl ester group to allow for covalent binding to cellular components, providing for better retention. As with any reagent, one should empirically determine retention times for the cell type used.

Find additional tips, troubleshooting help, and resources within our Cell Analysis Support Center.

I am doing a Live/Dead assay using Calcein, AM, for live cells and ethidium homodimer-1 for dead cells. Can I fix the cells after labeling and retain the staining?

This is not recommended. Neither Calcein nor ethidium homodimer-1 bind to any cellular components upon fixation. There is no guarantee that the dyes will be retained upon fixation or any subsequent wash steps. We recommend scoring for live and dead cells as soon as possible after staining.

Find additional tips, troubleshooting help, and resources within our Cell Analysis Support Center.

I need a general cytoplasmic stain that does not overlap with the GFP in my cells. What do you recommend?

Calcein AM, a green dye, is typically used as a general cytoplasmic stain, but not recommended with GFP-positive cells. For GFP-expressing cells there are other colors available: Calcein Blue AM, Calcein Violet AM, and Calcein Red-Orange AM. The retention time of these dyes in live cells is dependent upon the inherent properties of the cell.

Find additional tips, troubleshooting help, and resources within our Cell Analysis Support Center.

View all Calceína, AM, colorante de color persignificado celular FAQs

Citations & References (658)

Citations & References
Abstract
Nitric oxide mediates natural polyphenol-induced Bcl-2 down-regulation and activation of cell death in metastatic B16 melanoma.
Authors:Ferrer P,Asensi M,Priego S,Benlloch M,Mena S,Ortega A,Obrador E,Esteve JM,Estrela JM
Journal:The Journal of biological chemistry
PubMed ID:17135264
Cloning and expression of murine sister of P-glycoprotein reveals a more discriminating transporter than MDR1/P-glycoprotein.
Authors:Lecureur V,Sun D,Hargrove P,Schuetz EG,Kim RB,Lan LB,Schuetz JD
Journal:Molecular pharmacology
PubMed ID:10617675
Deposition of laminin 5 by keratinocytes regulates integrin adhesion and signaling.
Authors:Nguyen BP,Gil SG,Carter WG
Journal:The Journal of biological chemistry
PubMed ID:10926936
Large-scale chemical dissection of mitochondrial function.
Authors:Wagner BK,Kitami T,Gilbert TJ,Peck D,Ramanathan A,Schreiber SL,Golub TR,Mootha VK
Journal:Nature biotechnology
PubMed ID:18297058
Mitochondrial oxidative phosphorylation (OXPHOS) is central to physiology and disease pathogenesis. To systematically investigate its activity and regulation, we performed a wide range of assays of OXPHOS physiology and nuclear and mitochondrial gene expression across 2490 chemical perturbations in muscle cells. Through mining of the resulting compendium, we discovered that: ... More
Modification of the cytoplasmic domain of influenza virus hemagglutinin affects enlargement of the fusion pore.
Authors:Kozerski C,Ponimaskin E,Schroth-Diez B,Schmidt MF,Herrmann A
Journal:Journal of virology
PubMed ID:10906206
The fusion activity of chimeras of influenza virus hemagglutinin (HA) (from A/fpv/Rostock/34; subtype H7) with the transmembrane domain (TM) and/or cytoplasmic tail (CT) either from the nonviral, nonfusogenic T-cell surface protein CD4 or from the fusogenic Sendai virus F-protein was studied. Wild-type or chimeric HA was expressed in CV-1 cells ... More
View all Calceína, AM, colorante de color persignificado celular citations