Metabolic labeling of glycans with azido sugars for visualization and glycoproteomics.
AuthorsLaughlin ST, Agard NJ, Baskin JM, Carrico IS, Chang PV, Ganguli AS, Hangauer MJ, Lo A, Prescher JA, Bertozzi CR,
JournalMethods Enzymol
PubMed ID17116478
'The staggering complexity of glycans renders their analysis extraordinarily difficult, particularly in living systems. A recently developed technology, termed metabolic oligosaccharide engineering, enables glycan labeling with probes for visualization in cells and living animals, and enrichment of specific glycoconjugate types for proteomic analysis. This technology involves metabolic labeling of glycans ... More
Dynamic monitoring of newly synthesized proteomes: up-regulation of myristoylated protein kinase A during butyric acid induced apoptosis.
AuthorsLiu K, Yang PY, Na Z, Yao SQ,
JournalAngew Chem Int Ed Engl
PubMed ID21678537
Doubly charged: A double metabolic incorporation approach capable of proteome-wide profiling of post-translational modification dynamics on newly synthesized proteins has been developed (see scheme; blue box: methionine surrogate, orange diamond: PTM probe). This strategy reveals for the first time that up-regulation of myristoylated PKA protein is necessary for the occurrence ... More
The chemical neurobiology of carbohydrates.
AuthorsMurrey HE, Hsieh-Wilson LC,
JournalChem Rev
PubMed ID18452339
The problems associated with oligosaccharide analysis have hindered efforts to understand the biology of oligosaccharides yet have given chemists a unique opportunity to develop new methods to overcome these challenges. The development of chemical tools for the analysis of glycan structure and function is essential to advance our understanding of ... More
A functional RNAi screen links O-GlcNAc modification of ribosomal proteins to stress granule and processing body assembly.
AuthorsOhn T, Kedersha N, Hickman T, Tisdale S, Anderson P,
JournalNat Cell Biol
PubMed ID18794846
Stress granules (SGs) and processing bodies (PBs) are microscopically visible ribonucleoprotein granules that cooperatively regulate the translation and decay of messenger RNA. Using an RNA-mediated interference-based screen, we identify 101 human genes required for SG assembly, 39 genes required for PB assembly, and 31 genes required for coordinate SG and ... More