Invitrogen™

Click-IT™ Tetramethylrhodamine (TAMRA) Protein Analysis Detection Kit This product, and/or its packaging, incorporates one or more environmental/green features. Click on this icon to learn more.

Catalog number:  C33370

Related applications:

Labeling Chemistry

| Protein Electrophoresis & Western Blotting

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Description

The Click-iT® Tetramethylrhodamine (TAMRA) Glycoprotein Detection Kit provides the second part of the simple and robust two-step technique to identify and characterize glycoproteins by one- or two-dimensional gel electrophoresis. In step two, after the incorporation of the azide handle into protein glycan structures with either a Click-iT® metabolic labeling reagent or the Click-iT® Enzymatic Labeling system, the azide-modified glycoproteins are detected via the chemoselective ligation or click reaction between an azide and an alkyne. Gels with TAMRA labeled glycoproteins can be subsequently stained with the Multiplexed Proteomics™ technologies, SYPRO® Ruby total protein stain and PRO-Q® Emerald glycoprotein stain for the differential analysis of glycoprotein subclasses, total proteins and total glycoproteins in the same gel. Click-iT® modified glycoproteins are also compatible with downstream LC-MS/MS and MALDI MS analysis for identification.
For Research Use Only. Not for use in diagnostic procedures.

Specifications

Labeling Target: Proteins (Glycoproteins)
Shipping Condition: Room Temperature
Product Line: Click-iT™
Detection Method: Fluorescent
Label or Dye: TAMRA Isomers, TMR (Tetramethylrhodamine)
Green Features: Less hazardous

Contents & storage

Store in freezer (-5 to -30°C).