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      Invitrogen™

      CellTrace™ Oregon Green™ 488 Carboxylic Acid Diacetate, Succinimidyl Ester (Carboxy-DFFDA, SE), cell permeant, mixed isomers

      CellTrace™ Oregon Green™ 488 Carboxylic Acid Diacetate, Succinimidyl Ester (Carboxy-DFFDA, SE), cell permeant, mixed isomers

      Catalog number:  C34555

       Related applications: Cell Tracing & Tracking | Cell Viability, Proliferation & Function | Cellular Imaging | Pharma & Biopharma | Target-Based ADME⁄Tox Assays

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      Description

      Like CFDA SE, CellTrace™ Oregon Green® 488 (carboxy-DFFDA SE) should be a useful tool for following proliferating cells. This Oregon Green® 488 probe passively diffuses into cells, where it is colorless and nonfluorescent until its acetate groups are removed by intracellular esterases to yield a highly fluorescent, amine-reactive dye. Upon reaction with intracellular amines, the probe forms Oregon Green® 488 conjugates that are well-retained by cells. Unlike fluorescein conjugates, Oregon Green® 488 conjugates exhibit bright, photostable green fluorescence that is independent of pH at typical cellular pH values (pH-6-8).
      For Research Use Only. Not for use in diagnostic procedures.

      Specifications

      Reagent Type: Cell Labeling Reagents, Cell Tracker Compounds
      Detection Method: Fluorescent
      Excitation⁄Emission (nm): 496⁄524, 497⁄592
      Flow Cytometer Laser Lines: 488
      For Use With (Equipment): Flow Cytometer, Fluorescence Microscope
      Form: Solid
      Label or Dye: Oregon Green® 488
      Product Line: CellTrace™, Oregon Green®
      Product Size: 20 x 50 µg
      Solubility: DMSO (Dimethylsulfoxide)
      Shipping Condition: Room Temperature

      Contents & storage

      Contains 20 vials of Oregon Green® 488 carboxylic acid diacetate, SE (50 µg/vial).

      Store in freezer (-5 to -30°C) and protect from light.

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