Prepare stock solutions with confidence using fresh, single-use size anhydrous DMSO (order D12345)
Invitrogen™
CellTrace™ Oregon Green™ 488 Carboxylic Acid Diacetate, Succinimidyl Ester (Carboxy-DFFDA, SE), cell permeant, mixed isomers
Description
Like CFDA SE, CellTrace™ Oregon Green® 488 (carboxy-DFFDA SE) should be a useful tool for following proliferating cells. This Oregon Green® 488 probe passively diffuses into cells, where it is colorless and nonfluorescent until its acetate groups are removed by intracellular esterases to yield a highly fluorescent, amine-reactive dye. Upon reaction with intracellular amines, the probe forms Oregon Green® 488 conjugates that are well-retained by cells. Unlike fluorescein conjugates, Oregon Green® 488 conjugates exhibit bright, photostable green fluorescence that is independent of pH at typical cellular pH values (pH-6-8).
For Research Use Only. Not for use in diagnostic procedures.
Specifications
| Excitation/Emission: | 496⁄524, 497⁄592 |
|---|---|
| Form: | Solid |
| Flow Cytometer Laser Lines: | 488 |
| Reagent Type: | Cell Tracker Compounds, Cell Labeling Reagents |
| Solubility: | DMSO (Dimethylsulfoxide) |
| Shipping Condition: | Room Temperature |
| Product Line: | CellTrace™, Oregon Green® |
| Detection Method: | Fluorescent |
| Label or Dye: | Oregon Green® 488 |
| Label Type: | Classic Dyes |
| For Use With (Equipment): | Fluorescence Microscope, Flow Cytometer |
Contents & storage
Contains 20 vials of Oregon Green® 488 carboxylic acid diacetate, SE (50 µg/vial).
Store in freezer (-5 to -30°C) and protect from light.
Store in freezer (-5 to -30°C) and protect from light.
