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CellTrace™ Oregon Green® 488 Carboxylic Acid Diacetate, Succinimidyl Ester (Carboxy-DFFDA, SE), cell permeant, mixed isomers

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Description

Like CFDA SE, CellTrace™ Oregon Green® 488 (carboxy-DFFDA SE) should be a useful tool for following proliferating cells. This Oregon Green® 488 probe passively diffuses into cells, where it is colorless and nonfluorescent until its acetate groups are removed by intracellular esterases to yield a highly fluorescent, amine-reactive dye. Upon reaction with intracellular amines, the probe forms Oregon Green® 488 conjugates that are well-retained by cells. Unlike fluorescein conjugates, Oregon Green® 488 conjugates exhibit bright, photostable green fluorescence that is independent of pH at typical cellular pH values (pH-6-8).
For Research Use Only. Not for use in diagnostic procedures.

Specifications

Reagent Type: Cell Labeling Reagents, Cell Tracker Compounds
Detection Method: Fluorescent
Excitation⁄Emission (nm): 496⁄524, 497⁄592
Flow Cytometer Laser Lines: 488
For Use With (Equipment): Flow Cytometer, Fluorescence Microscope
Form: Solid
Label or Dye: Oregon Green® 488
Product Size: 20 x 50 µg
Solubility: DMSO (Dimethylsulfoxide)
Shipping Condition: Room Temperature

Contents & storage

Contains 20 vials of Oregon Green® 488 carboxylic acid diacetate, SE (50 µg/vial).

Store in freezer (-5 to -30°C) and protect from light.

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