Invitrogen™

CellTrace™ Yellow Cell Proliferation Kit, for flow cytometry

Catalog number:  C34573

 Related applications:

Flow Cytometry

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Description

CellTrace™ Yellow Cell Proliferation Kit is used for in vitro and in vivo labeling of cells to trace multiple generations using dye dilution by flow cytometry. This reagent is specifically designed for excitation by either a 532-nm or 561-nm laser, and it can be easily multiplexed with reagents and antibodies excited by other commonly used lasers.

Superior performance—bright, single-peak staining enables visualization of multiple generations
Long-term signal stability—well retained in cells for several days post stain
Versatile—multiple colors available to easily combine with antibodies or markers of cell function
Simple—robust staining protocol

Additional information is available about other CellTrace™ Reagents to meet your multiplexing needs.

Superior fluorescent staining
Successful proliferation analysis by dye dilution requires an extremely bright dye to distinguish fluorescently labeled cells from auto-fluorescence after several cell divisions. The intense fluorescent staining provided by CellTrace Yellow dye enables the visualization of six or more generations of proliferating cells before the signal is overwhelmed by intrinsic cellular auto-fluorescence. This kit enables consistent, homogeneous staining results with little fluorescence variation between cells in a population, so distinct generations can be seen without any requirement for complex modeling software.

Long-term signal retention
Unlike stains that label the lipid membrane of cells, CellTrace Yellow stain easily crosses the plasma membrane and covalently binds inside cells where the stable, well-retained fluorescent dye offers a consistent signal, even after several days in a cell culture environment. CellTrace Yellow dye binds covalently to all free amines on the surface and inside of cells and shows little cytotoxicity, with minimal observed effect on the proliferative ability or biology of cells.

Easy multiplexing with other fluorophores
The yellow excitation at 546 nm and emission at 579 nm of CellTrace Yellow dye make it ideal for multiplexing due to the limited spectral overlap with other common dyes (e.g., Alexa Fluor™488 and FITC) and green fluorescent protein (GFP) .

Use the SpectraViewer to help you plan your experiments.

Simple, robust staining protocol
The CellTrace Yellow Cell Proliferation Kit contains convenient single-use vials of dry dye to permit small-scale experiments without preparing excess quantities of dye. A stock solution is prepared by dissolving the contents of a vial in anhydrous DMSO prior to use. To stain 1 mL of cells in protein-free medium, 1 µL of this stock solution is typically used. Cells should be stained for 20 minutes at room temperature with gentle agitation. A brief wash with complete medium will then quench any dye remaining in solution.

Related links
General information about Thermo Fisher Scientific flow cytometry products and support is also available.
For Research Use Only. Not for use in diagnostic procedures.

Specifications

Flow Cytometer Laser Lines: 532, 532 or 561
For Use With (Equipment): Flow Cytometer
Detection Method: Fluorescent
Format: Tube(s)
Excitation⁄Emission (nm): 546⁄579
Form: Solid
Label or Dye: CellTrace™ Yellow
Number of Reactions: 20 Reactions
Product Line: CellTrace™
Product Size: 20 assays
Shipping Condition: Room Temperature

Contents & storage

• 1 vial CellTrace™ Yellow Dye
• 100 µL DMSO

Store in freezer (-5 to -30°C).

Documents

Manuals & protocols