Invitrogen™

CellTrace™ Yellow Cell Proliferation Kit, for flow cytometry

Catalog number: C34573
Invitrogen™

CellTrace™ Yellow Cell Proliferation Kit, for flow cytometry

Catalog number: C34573
Catalog Number
C34573
Unit Size
20 assays
Price (USD)
105.00
Your Price:
Availability
***
Quantity
Catalog Number
Unit Size
180 assays
Price (USD)
401.00
Your Price:
Availability
***
Quantity
Catalog NumberUnit SizePrice (USD)AvailabilityQuantity
C3457320 assays
105.00
Your Price:
***
C34567180 assays
401.00
Your Price:
***
Product Overview
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CellTrace™ Yellow Cell Proliferation Kit is used for in vitro and in vivo labeling of cells to trace multiple generations using dye dilution by flow cytometry. This reagent is specifically designed for excitation by either a 532-nm or 561-nm laser, and it can be easily multiplexed with reagents and antibodies excited by other commonly used lasers.

Superior performance—bright, single-peak staining enables visualization of multiple generations
Long-term signal stability—well retained in cells for several days post stain
Versatile—multiple colors available to easily combine with antibodies or markers of cell function
Simple—robust staining protocol

Superior fluorescent staining
Successful proliferation analysis by dye dilution requires an extremely bright dye to distinguish fluorescently labeled cells from auto-fluorescence after several cell divisions. The intense fluorescent staining provided by CellTrace Yellow dye enables the visualization of six or more generations of proliferating cells before the signal is overwhelmed by intrinsic cellular auto-fluorescence. This kit enables consistent, homogeneous staining results with little fluorescence variation between cells in a population, so distinct generations can be seen without any requirement for complex modeling software.

Long-term signal retention
Unlike stains that label the lipid membrane of cells, CellTrace Yellow stain easily crosses the plasma membrane and covalently binds inside cells where the stable, well-retained fluorescent dye offers a consistent signal, even after several days in a cell culture environment. CellTrace Yellow dye binds covalently to all free amines on the surface and inside of cells and shows little cytotoxicity, with minimal observed effect on the proliferative ability or biology of cells.

Easy multiplexing with other fluorophores
The yellow excitation at 546 nm and emission at 579 nm of CellTrace Yellow dye make it ideal for multiplexing due to the limited spectral overlap with other common dyes (e.g., Alexa Fluor™488 and FITC) and green fluorescent protein (GFP) .

Use the SpectraViewer to help you plan your experiments.

Simple, robust staining protocol
The CellTrace Yellow Cell Proliferation Kit contains convenient single-use vials of dry dye to permit small-scale experiments without preparing excess quantities of dye. A stock solution is prepared by dissolving the contents of a vial in anhydrous DMSO prior to use. To stain 1 mL of cells in protein-free medium, 1 µL of this stock solution is typically used. Cells should be stained for 20 minutes at room temperature with gentle agitation. A brief wash with complete medium will then quench any dye remaining in solution.

For Research Use Only. Not for use in diagnostic procedures.

Specifications

Detection Method
Fluorescent
Dye Type
CellTrace™ Yellow
Emission
532 or 561, 532
Form
Solid
Format
Tube(s)
Product Line
CellTrace™
Quantity
20 Assays
Reagent Type
CFSE & Related Compounds
Shipping Condition
Room Temperature
Solubility
DMSO (Dimethylsulfoxide)
For Use With (Equipment)
Flow Cytometer

Contents & Storage

  • 1 vial CellTrace™ Yellow Dye
  • 100 µL DMSO
  • Store in freezer (-5°C to -30°C)

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Frequently asked questions (FAQs)

Citations & References