CellTrace™ Calcein Violet, AM, for 405 nm excitation - Special Packaging

Citas y referencias (5)

Invitrogen™

CellTrace™ Calcein Violet, AM, for 405 nm excitation - Special Packaging

La calceína violeta AM CellTrace™ es un colorante óptimo para distinguir las células vivas por la presencia de actividad deMás información

Número de catálogo	Cantidad
C34858	1.000 reacciones

Número de catálogo C34858

Precio (MXN)

Cantidad:

1.000 reacciones

La calceína violeta AM CellTrace™ es un colorante óptimo para distinguir las células vivas por la presencia de actividad de esterasa intracelular ubicua. La conversión enzimática de la calceína violeta AM que penetra en las células, prácticamente no fluorescente, en calceína violeta, intensamente fluorescente (ex/em 400/452 nm), puede detectarse fácilmente con el láser violeta, lo que permite utilizar otras líneas láser para los fluorocromos convencionales.

Consulte la guía de selección para todos los colorantes de viabilidad no fijables para citometría de flujo.

Para uso exclusivo en investigación. No apto para uso en procedimientos diagnósticos.

Especificaciones

Método de detecciónFluorescente

Tipo de colorantecalceína violeta

Intervalo de longitud de onda de excitación400⁄452

Para utilizar con (equipo)Citómetro de flujo

FormatoTubo(s)

Línea de productosCellTrace

Cantidad1.000 reacciones

Tipo de reactivoCompuestos de seguimiento celular, reactivos de etiquetado celular

Condiciones de envíoTemperatura ambiente

Tipo de productoTinte

Unit SizeEach

Contenido y almacenamiento

Contiene 20 viales de calceína violeta AM (25 μg por vial). Almacenar en el congelador (de – 5 a – 30 °C) y proteger de la luz.

Preguntas frecuentes

I need a general cytoplasmic stain that does not overlap with the GFP in my cells. What do you recommend?

Calcein AM, a green dye, is typically used as a general cytoplasmic stain, but not recommended with GFP-positive cells. For GFP-expressing cells there are other colors available: Calcein Blue AM, Calcein Violet AM, and Calcein Red-Orange AM. The retention time of these dyes in live cells is dependent upon the inherent properties of the cell.

Find additional tips, troubleshooting help, and resources within our Cell Analysis Support Center.

I stained two populations of cells, one with CellTracker Green and the other with CellTracker Red, but it looks like there may be crossover of the red dye to the green cells. What is going on?

One possibility is that there is spectral bleedthrough between the dyes. Be sure to check the single-color samples by imaging the red cells in green and imaging the green cells in red, using the optimal imaging settings for the other color. If you see bleedthrough with these controls, then you will have to reduce the dye label concentration to reduce the brightness of the dyes, or choose dyes that are farther apart spectrally. If the issue isn’t bleedthrough, another possibility is that the cells were not adequately washed after staining, allowing some unincorporated dye to remain and label the other cells after they were introduced. Extending washes and wash times should help with this.

Find additional tips, troubleshooting help, and resources within our Cell Analysis Support Center.

I stained my cells with Calcein, AM, but the signal went away after I fixed my cells. Why is this?

Calcein, AM diffuses into cells, the 'AM' moiety is cleaved by cellular esterases, and then the dye molecules are observed in the cytoplasm without binding to anything. This gives a 'whole cell' stain. It also means that the dyes are not crosslinked with aldehyde-based fixation and thus will be lost upon fixation. Additionally, any disruption of plasma membrane, such as with detergents or trypsinization, will lead to leakage of the dye from the cell.

Find additional tips, troubleshooting help, and resources within our Cell Analysis Support Center.

I'm trying to stain my cells with CellTracker dyes or CFDA SE, but I'm not seeing much signal. What can I do?

First, make sure you aren’t staining in the presence of serum, since serum can have esterase activity that can prematurely cleave the AM group on these dyes, preventing entry into cells. After staining, it’s okay to return the cells to medium containing serum. After this, you can try increasing the concentration and label time to get a higher intensity.

Find additional tips, troubleshooting help, and resources within our Cell Analysis Support Center.

I like how calcein dyes label the whole cell. How long can I track my cells with them, and can I fix them?

Calcein dyes diffuse into cells, the 'AM' moiety is cleaved by cellular esterases and then are observed in the cytoplasm without binding to anything. This provides a 'whole cell' label. Calcein dyes may be pumped out by normal cellular efflux mechanisms, sometimes within a very short time, especially for cell types that may exhibit drug resistance, unless the efflux is inhibited (such as with probenecid). The dyes are not crosslinked with aldehyde-based fixation, unlike protein-binding CellTracker dyes, and thus will be lost upon fixation. Additionally, any disruption of plasma membrane, such as with detergents or trypsinization, will lead to leakage of the dyes from the cell.

Find additional tips, troubleshooting help, and resources within our Cell Tracing and Tracking Support Center.

View all CellTrace™ Calcein Violet, AM, for 405 nm excitation - Special Packaging FAQs

Citations & References (5)

Citations & References

Abstract

Toll-like receptor 2-mediated signaling requirements for Francisella tularensis live vaccine strain infection of murine macrophages.

Authors:Cole LE, Shirey KA, Barry E, Santiago A, Rallabhandi P, Elkins KL, Puche AC, Michalek SM, Vogel SN,

Journal:Infect Immun

PubMed ID:17517865

'Francisella tularensis, an aerobic, non-spore-forming, gram-negative coccobacillus, is the causative agent of tularemia. We reported previously that F. tularensis live vaccine strain (LVS) elicited strong, dose-dependent NF-kappaB reporter activity in Toll-like receptor 2 (TLR2)-expressing HEK293T cells and proinflammatory gene expression in primary murine macrophages. Herein, we report that F. tularensis ... More

Trogocytosis by Entamoeba histolytica contributes to cell killing and tissue invasion.

Authors:Ralston KS, Solga MD, Mackey-Lawrence NM, Somlata, Bhattacharya A, Petri WA,

Journal:

PubMed ID:24717428

'Entamoeba histolytica is the causative agent of amoebiasis, a potentially fatal diarrhoeal disease in the developing world. The parasite was named '

Bicarbonate and functional CFTR channel are required for proper mucin secretion and link cystic fibrosis with its mucus phenotype.

Authors:Gustafsson JK, Ermund A, Ambort D, Johansson ME, Nilsson HE, Thorell K, Hebert H, Sjövall H, Hansson GC,

Journal:J Exp Med

PubMed ID:22711878

'Cystic fibrosis (CF) is caused by a nonfunctional chloride and bicarbonate ion channel (CF transmembrane regulator [CFTR]), but the link to the phenomenon of stagnant mucus is not well understood. Mice lacking functional CFTR (Cftr?508) have no lung phenotype but show similar ileal problems to humans. We show that the ... More

Multiplexed labeling of viable cells for high-throughput analysis of glycine receptor function using flow cytometry.

Authors:Gilbert DF, Wilson JC, Nink V, Lynch JW, Osborne GW,

Journal:Cytometry A

PubMed ID:19184990

Flow cytometry is an important drug discovery tool because it permits high-content multiparameter analysis of individual cells. A new method dramatically enhanced screening throughput by multiplexing many discrete fixed cell populations; however, this method is not suited to assays requiring functional cellular responses. HEK293 cells were transfected with unique mutant ... More

Induction of heat shock proteins in cerebral cortical cultures by celastrol.

Authors:Chow AM, Tang DW, Hanif A, Brown IR

Journal:Cell Stress Chaperones

PubMed ID:22865541

Alzheimer's disease, Parkinson's disease and amyotrophic lateral sclerosis (ALS) are 'protein misfolding disorders' of the mature nervous system that are characterized by the accumulation of protein aggregates and selective cell loss. Different brain regions are impacted, with Alzheimer's affecting cells in the cerebral cortex, Parkinson's targeting dopaminergic cells in the ... More