JournalClinical cancer research : an official journal of the American Association for Cancer Research
PubMed ID17545527
Functional symbiosis between endothelium and epithelial cells in glomeruli.
AuthorsHirschberg R, Wang S, Mitu GM,
JournalCell Tissue Res
PubMed ID17999087
'In some capillary beds, pericytes regulate endothelial growth. Capillaries with high filtration capacity, such as those in renal glomeruli, lack pericytes. Glomerular endothelium lies adjacent to visceral epithelial cells (podocytes) that are anchored to and cover the anti-luminal surface of the basement membrane. We have tested the hypothesis that podocytes ... More
Flex-Hets differentially induce apoptosis in cancer over normal cells by directly targeting mitochondria.
AuthorsLiu T, Hannafon B, Gill L, Kelly W, Benbrook D
JournalMol Cancer Ther
PubMed ID17575110
'Flex-Het drugs induce apoptosis in multiple types of cancer cells, with little effect on normal cells. This apoptosis occurs through the intrinsic mitochondrial pathway accompanied by generation of reactive oxygen species (ROS). The objective of this study was to determine if direct or indirect targeting of mitochondria is responsible for ... More
Cytosolic delivery of membrane-impermeable molecules in dendritic cells using pH-responsive core-shell nanoparticles.
AuthorsHu Y, Litwin T, Nagaraja AR, Kwong B, Katz J, Watson N, Irvine DJ,
JournalNano Lett
PubMed ID17887715
'Polycations that absorb protons in response to the acidification of endosomes can theoretically disrupt these vesicles via the "proton sponge" effect. To exploit this mechanism, we created nanoparticles with a segregated core-shell structure for efficient, noncytotoxic intracellular drug delivery. Cross-linked polymer nanoparticles were synthesized with a pH-responsive core and hydrophilic ... More
Decrease of endogenous vascular endothelial growth factor may not affect glioma cell proliferation and invasion.
'Vascular endothelial growth factor (VEGF) is abundantly produced by glioma cells especially glioblastoma, the most malignant form of astrocytoma. VEGF, a well known angiogenic factor, acts in a paracrine fashion on endothelial cells to develop tumor vasculature. However, recent studies have found that several tumor cells express VEGF receptors, and ... More
Application of a high-content multiparameter cytotoxicity assay to prioritize compounds based on toxicity potential in humans.
AuthorsAbraham VC, Towne DL, Waring JF, Warrior U, Burns DJ,
JournalJ Biomol Screen
PubMed ID18566484
'Prioritization of compounds based on human hepatotoxicity potential is currently a key unmet need in drug discovery, as it can become a major problem for several lead compounds in later stages of the drug discovery pipeline. The authors report the validation and implementation of a high-content multiparametric cytotoxicity assay based ... More
HYAL1 and HYAL2 inhibit tumour growth in vivo but not in vitro.
AuthorsWang F, Grigorieva EV, Li J, Senchenko VN, Pavlova TV, Anedchenko EA, Kudryavtseva AV, Tsimanis A, Angeloni D, Lerman MI, Kashuba VI, Klein G, Zabarovsky ER,
JournalPLoS ONE
PubMed ID18725949
'BACKGROUND: We identified two 3p21.3 regions (LUCA and AP20) as most frequently affected in lung, breast and other carcinomas and reported their fine physical and gene maps. It is becoming increasingly clear that each of these two regions contains several TSGs. Until now TSGs which were isolated from AP20 and ... More
Neutrophil elastase cleaves VEGF to generate a VEGF fragment with altered activity.
AuthorsKurtagic E, Jedrychowski MP, Nugent MA,
JournalAm J Physiol Lung Cell Mol Physiol
PubMed ID19136576
'Excessive neutrophil elastase (NE) activity and altered vascular endothelial growth factor (VEGF) signaling have independently been implicated in the development and progression of pulmonary emphysema. In the present study, we investigated the potential link between NE and VEGF. We noted that VEGF(165) is a substrate for NE. Digestion of purified ... More
Methoxychlor and estradiol induce oxidative stress DNA damage in the mouse ovarian surface epithelium.
AuthorsSymonds DA, Merchenthaler I, Flaws JA,
JournalToxicol Sci
PubMed ID18502743
'Estrogenic compounds such as 17beta-estradiol (E(2)) and methoxychlor (MXC) induce oxidative stress damage in breast cells and mouse ovarian follicles, respectively. However, little is known about whether estrogenic compounds cause oxidative stress in the ovarian surface epithelium (OSE). Thus, this work tested the hypothesis that E(2) and MXC cause oxidative ... More
Reductively labile PRINT particles for the delivery of doxorubicin to HeLa cells.
AuthorsPetros RA, Ropp PA, DeSimone JM,
JournalJ Am Chem Soc
PubMed ID18355010
A Trojan horse PRINT particle composition was developed that incorporates a reductively labile cross-linker to achieve activated release of doxorubicin in vitro. Particles of discrete size and shape (cube side length = 2 micron) containing 30 wt % of a disulfide-based cross-linker and 2 wt % doxorubicin were synthesized. This ... More
Quinotrierixin inhibits proliferation of human retinal pigment epithelial cells.
AuthorsChen C, Wang JJ, Li J, Yu Q, Zhang SX,
JournalMol Vis
PubMed ID23335849
To investigate the effect of quinotrierixin, a previously reported inhibitor of X-box binding protein 1 (XBP1), on cell proliferation and viability in human retinal pigment epithelium (RPE) cells. Subconfluent human RPE cells (ARPE-19) were exposed to quinotrierixin for 16-24 h. Cell proliferation was determined with 3-(4, 5-dimethylthiazolyl-2)-2,5-diphenyltetrazolium bromide assay, hemocytometer ... More
Prion strain discrimination in cell culture: the cell panel assay.
AuthorsMahal SP, Baker CA, Demczyk CA, Smith EW, Julius C, Weissmann C,
JournalProc Natl Acad Sci U S A
PubMed ID18077360
Prions are thought to consist mainly or entirely of misfolded PrP, a constitutively expressed host protein. Prions associated with the same PrP sequence may occur in the form of different strains; the strain phenotype is believed to be encoded by the conformation of the PrP. Some cell lines can be ... More
1-Benzyl-3-cetyl-2-methylimidazolium iodide (NH125) induces phosphorylation of eukaryotic elongation factor-2 (eEF2): a cautionary note on the anticancer mechanism of an eEF2 kinase inhibitor.
AuthorsChen Z, Gopalakrishnan SM, Bui MH, Soni NB, Warrior U, Johnson EF, Donnelly JB, Glaser KB,
JournalJ Biol Chem
PubMed ID22020937
Eukaryotic elongation factor-2 kinase (eEF2K) relays growth and stress signals to protein synthesis through phosphorylation and inactivation of eukaryotic elongation factor 2 (eEF2). 1-Benzyl-3-cetyl-2-methylimidazolium iodide (NH125) is a widely accepted inhibitor of mammalian eEF2K and an efficacious anti-proliferation agent against different cancer cells. It implied that eEF2K could be an ... More
Endogenous heparan sulfate and heparin modulate bone morphogenetic protein-4 signaling and activity.
AuthorsKhan SA, Nelson MS, Pan C, Gaffney PM, Gupta P,
JournalAm J Physiol Cell Physiol
PubMed ID18385288
Bone morphogenetic proteins (BMPs) and their endogenous antagonists are important for brain and bone development and tumor initiation and progression. Heparan sulfate (HS) proteoglycans (HSPG) modulate the activities of BMPs and their antagonists. How glycosaminoglycans (GAGs) influence BMP activity in various malignancies and in inherited abnormalities of GAG metabolism, and ... More
Lysyl oxidase oxidizes cell membrane proteins and enhances the chemotactic response of vascular smooth muscle cells.
Lysyl oxidase (LOX) is a potent chemokine inducing the migration of varied cell types. Here we demonstrate that inhibition of LOX activity by beta-aminopropionitrile (BAPN) in cultured rat aortic smooth muscle cells (SMCs) reduced the chemotactic response and sensitivity of these cells toward LOX and toward PDGF-BB. The chemotactic activity ... More