CyQUANT™ NF Cell Proliferation Assay, 1000 assays - FAQs

View additional product information for CyQUANT™ Cell Proliferation Assays - FAQs (C35012, C35013, C35011, C7027, C7026, C35006, C35007)

3 product FAQs found

What is the difference between the original CyQuant assay and the CyQuant NF assay?

The original CyQUANT assay provides sensitive detection of cells over a 1000-fold linear dynamic range. In this assay, a freeze-thaw cell lysis step is required to facilitate the interaction of the CyQUANT GR dye with DNA. The CyQUANT NF assay avoids this freeze-thaw step by using a DNA binding dye in combination with a plasma membrane permeabilization reagent. The CyQUANT NF protocol requires only aspiration of growth medium (for adherent cells), replacement with dye binding solution, incubation for 30-60 minutes, and then measurement of fluorescence in a microplate reader. The assay is designed to produce a linear analytical response from at least 100-20,000 cells per well in most cell lines in a 96-well microplate.

Find additional tips, troubleshooting help, and resources within our Cell Analysis Support Center.

Do you have spectral data for the CyQUANT NF Cell Proliferation Assay kit?

When CyQUANT NF dye is bound to DNA, the approximate excitation/emission is 480/520 nm. It can be detected using standard GFP or FITC filter settings.

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Do you offer any products to measure neuronal cell health?

PrestoBlue Cell Viability Stain and CyQUANT Cell Proliferation Assay Kit can be used. We also offer a Neurite Outgrowth Staining Kit (Cat. No. A15001). More information about our different assays for neuronal cell health can be found here (https://www.thermofisher.com/us/en/home/life-science/cell-analysis/neuroscience/neuronal-cell-health-assays.html).

Find additional tips, troubleshooting help, and resources within our Cell Analysis Support Center.