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CyQUANT™ NF Zellproliferations-Assay
Zitierungen und Referenzen (17)
Invitrogen™

CyQUANT™ NF Zellproliferations-Assay

Der CyQUANT™ NF Zellproliferations-Assay ist eine schnelle und empfindliche Methode zur Zellzählung in einer Population und zur Messung der ProliferationWeitere Informationen
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KatalognummerZelltypMenge
C35012Direct Cell100 Microplates
C35011Direct Cell10 Microplates
C35013Direct Red Cell10 Mikrotiterplatten
C35007NF Cell200 Assays
C7026For cells in culture1000 Assays
C35006NF Cell1000 Assays
C7027Cell Lysis Buffer50 ml
Katalognummer C35012
Preis (EUR)
2.854,65
Exklusiv online
3.005,00
Ersparnis 150,35 (5%)
Each
Zelltyp:
Direct Cell
Menge:
100 Microplates
Preis (EUR)
2.854,65
Exklusiv online
3.005,00
Ersparnis 150,35 (5%)
Each
Der CyQUANT™ NF Zellproliferations-Assay ist eine schnelle und empfindliche Methode zur Zellzählung in einer Population und zur Messung der Proliferation im Mikrotiterplattenformat.

Merkmale des CyQUANT™ NF Zellproliferations-Assay:

• Empfindlichere Assays als MTT- oder AlamarBlue™ Assays
• Linearer Nachweisbereich von 100 bis 20.000 Zellen pro Well (96-Well Mikrotiterplatte)
•Assays können in einer Stunde abgeschlossen werden

Schnelle und einfache Methode zur Messung der Zellproliferation
Der CyQUANT™ NF Zellproliferations-Assay erfordert keine Zelllyse, lange Inkubationen, Radioaktivität oder Entfernung von Färbemittel aus Zellen. Der CyQUANT™ NF-Assay macht den Einfrier- und Auftauschritt der Zelllyse des ursprünglichen CyQUANT™ Proliferationsassays hinfällig, indem ein zellmembrangängiger, DNA-bindender Farbstoff in Kombination mit einem Permeabilisationsreagenz für die Plasmamembran verwendet wird. Der CyQUANT™ NF Zellproliferationsassay kann im 96 Well- oder 384 Well-Mikrotiterplattenformat verwenden werden und ist in zwei Konfigurationen erhältlich: ein 200 Assay-Kit (C35007) für kleinere Probengrößen und ein 1.000 Assay-Kit (C35006) für Anwendungen mit hohem Durchsatz.

AlamarBlue™ ist ein eingetragenes Warenzeichen von TREK Diagnostic Systems.
Nur für Forschungszwecke. Nicht zur Verwendung bei diagnostischen Verfahren.
Specifications
ZelltypDirect Cell
KulturmilieuSuspensionszellkultur, adhärente Zellkultur
NachweisverfahrenFluoreszent, Fluoreszent
FarbstofftypAndere Etiketten oder Farbstoffe
Anregung/Emission508⁄527
Format96 Well-Platte, 1536 Well-Platte, 384 Well-Platte, 96-Well-Platte, 1536-Well-Platte, 384-Well-Platte
Inkubationszeit60 min, 60 min
Menge100 Microplates
VersandbedingungRaumtemperatur, Raumtemperatur
FarbeGrün
EmissionSichtbar
Zur Verwendung mit (Anwendung)Proliferationsassay
Zur Verwendung mit (Geräte)Mikrotiterplatten-Lesegerät, Spektralphotometer, Mikroskop, HTS-Lesegerät, Mikrotiterplatten-Lesegerät, Spektralphotometer, Mikroskop, HTS-Lesegerät
ProduktlinieCyQUANT, Molecular Probes
ProdukttypZellproliferationsassay
Unit SizeEach
Inhalt und Lagerung
  • 5 ml CyQUANT™ Direct Nukleinsäurefärbung (Komponente A)
  • 25 ml CyQUANT™ Direct Hintergrundsuppressor I (Komponente B)
  • Beide bei 2 °C bis 25 °C lagern. Mit Trockenmittel und vor Licht geschützt lagern.

Häufig gestellte Fragen (FAQ)

MTT cell proliferation plate assays require cellular metabolism to modify the reagent and thus, only live cells will be counted. Is this true for CyQUANT Direct Cell Proliferation Assay, too?

This is true for MTT (as well as XTT, AlamarBlue Cell Viability Reagent, and PrestoBlue Cell Viability Reagent). CyQUANT Direct will also only count live cells, but for a different reason. The dye in it is a green-fluorescent nucleic acid stain, which will bind to DNA in all cells, live or dead, without the need for cellular metabolism. However, there is a non-cell-permeable quenching reagent in the kit which will both quench extracellular fluorescence (and thus this is a no-wash assay) AND will quench the fluorescence in dead cells (but not live cells).

Find additional tips, troubleshooting help, and resources within our Cell Analysis Support Center.

MTT cell proliferation assays require cellular metabolism to turn over the reagent, and thus only live cells will be counted. Is this true for CyQUANT Direct as well?

This is true for MTT (as well as XTT, AlamarBlue Cell Viability Reagent, and PrestoBlue Cell Viability Reagent). CyQUANT Direct will also only count live cells, but for a different reason. The dye is a green-fluorescent nucleic acid stain, which will bind to DNA in all cells, live or dead, without the need for cellular metabolism. However, there is a cell impermeant quenching reagent in the kit which will quench both extracellular fluorescence (and thus this is a no-wash assay) and the fluorescence in dead cells (but not live cells).

Find additional tips, troubleshooting help, and resources within our Cell Analysis Support Center.

What volumes should I use to make a 10X stock solution for the CyQUANT Direct Cell Proliferation Assay (Cat. No. C35011, C35012)?

To make a 10X stock solution for the CyQUANT Direct Cell Proliferation Assay (Cat. No. C35011, C35012), we suggest using the following volumes:

1.1 mL         PBS
25 µL         CyQUANT Direct nucleic acid stain (Component A)
125 µL         CyQUANT Direct background suppressor I (Component B)

Find additional tips, troubleshooting help, and resources within our Cell Analysis Support Center.

Are the components of the CyQUANT Direct Red Cell Proliferation Assay and CyQuant Direct Cell Proliferation Assay kits interchangeable?

No. The components of the CyQUANT Direct Red Cell Proliferation Assay and CyQuant Direct Cell Proliferation Assay kits are not interchangeable.

Find additional tips, troubleshooting help, and resources within our Cell Analysis Support Center.

What is the difference between the CyQUANT Direct Red Cell Proliferation Assay (Cat. Nos. C35013, C35014) and the green fluorescent CyQuant Direct Cell Proliferation Assay (Cat Nos. C35011, C35012)?

The two kits utilize different dyes and background suppressors, but they function in the same way and provide similar results as can be seen from Figures 1 and 2 in the CyQUANT Direct Red Cell Proliferation Assay product sheet (https://assets.thermofisher.com/TFS-Assets/LSG/manuals/MAN0018644_CyQUANT_direct_red_assay_PI.pdf). Dyes from both kits are nucleic acid stains and the background suppressors are cell impermeant reagents that quench extracellular fluorescence. They are usable within the same range (100 to 20,000 cells/well in a 96-well format) and in the same workflow.

Find additional tips, troubleshooting help, and resources within our Cell Analysis Support Center.

View all CyQUANT™ NF Zellproliferations-Assay FAQs

Zitierungen und Referenzen (17)

Zitierungen und Referenzen
Abstract
Application of a high-content multiparameter cytotoxicity assay to prioritize compounds based on toxicity potential in humans.
Authors:Abraham VC, Towne DL, Waring JF, Warrior U, Burns DJ,
Journal:J Biomol Screen
PubMed ID:18566484
'Prioritization of compounds based on human hepatotoxicity potential is currently a key unmet need in drug discovery, as it can become a major problem for several lead compounds in later stages of the drug discovery pipeline. The authors report the validation and implementation of a high-content multiparametric cytotoxicity assay based ... More
Activator of G protein signaling 3 promotes epithelial cell proliferation in PKD.
Authors:Nadella R, Blumer JB, Jia G, Kwon M, Akbulut T, Qian F, Sedlic F, Wakatsuki T, Sweeney WE, Wilson PD, Lanier SM, Park F,
Journal:J Am Soc Nephrol
PubMed ID:20488951
'The activation of heterotrimeric G protein signaling is a key feature in the pathophysiology of polycystic kidney diseases (PKD). In this study, we report abnormal overexpression of activator of G protein signaling 3 (AGS3), a receptor-independent regulator of heterotrimeric G proteins, in rodents and humans with both autosomal recessive and ... More
The matricellular protein cysteine-rich protein 61 (CCN1/Cyr61) enhances physiological adaptation of retinal vessels and reduces pathological neovascularization associated with ischemic retinopathy.
Authors:Hasan A, Pokeza N, Shaw L, Lee HS, Lazzaro D, Chintala H, Rosenbaum D, Grant MB, Chaqour B,
Journal:J Biol Chem
PubMed ID:21212276
'Retinal vascular damages are the cardinal hallmarks of retinopathy of prematurity (ROP), a leading cause of vision impairment and blindness in childhood. Both angiogenesis and vasculogenesis are disrupted in the hyperoxia-induced vaso-obliteration phase, and recapitulated, although aberrantly, in the subsequent ischemia-induced neovessel formation phase of ROP. Yet, whereas the histopathological ... More
Selective blockade of cytoskeletal actin remodeling reduces experimental choroidal neovascularization.
Authors:Caballero S, Yang R, Grant MB, Chaqour B,
Journal:Invest Ophthalmol Vis Sci
PubMed ID:21178140
'The efficacy of the peptide Ac-EEED on reducing cell adhesion and proliferation in vitro and choroidal neovascularization (CNV) in vivo was examined. The peptide chimera containing the Ac-EEED sequence was chemically linked to the N terminus of the XMTM delivery peptide from the E(rns) viral surface protein. Ac-EEED or scrambled ... More
Loss of activator of G-protein signaling 3 impairs renal tubular regeneration following acute kidney injury in rodents.
Authors:Regner KR, Nozu K, Lanier SM, Blumer JB, Avner ED, Sweeney WE, Park F,
Journal:FASEB J
PubMed ID:21343176
The intracellular mechanisms underlying renal tubular epithelial cell proliferation and tubular repair following ischemia-reperfusion injury (IRI) remain poorly understood. In this report, we demonstrate that activator of G-protein signaling 3 (AGS3), an unconventional receptor-independent regulator of heterotrimeric G-protein function, influences renal tubular regeneration following IRI. In rat kidneys exposed to ... More
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