An impaired transendothelial migration potential of chronic lymphocytic leukemia (CLL) cells can be linked to ephrin-A4 expression.
AuthorsTrinidad EM, Ballesteros M, Zuloaga J, Zapata A, Alonso-Colmenar LM,
JournalBlood
PubMed ID19828693
'Chronic lymphocytic leukemia (CLL) cell migration into lymphoid tissues is an important aspect of the pathobiology of this disease. Here, we investigated the role of ephrin-A4 (EFNA4) in the transendothelial migration (TEM) capacity of CLL and normal B cells through interacting with endothelial EphA2 (erythropoietin-producing hepatocellular carcinoma). CLL cells showed ... More
Analysis of microRNA and protein transfer by exosomes during an immune synapse.
AuthorsVillarroya-Beltri C, Gutiérrez-Vázquez C, Sánchez-Madrid F, Mittelbrunn M,
Journal
PubMed ID23719941
'Immune cells release microRNA-containing exosomes that can be taken up by recipient cells. Exosomes can thus act as mediators of cell-cell communication through direct exchange of genetic material between cells. Exosome-mediated transfer of miRNAs between T cells and antigen-presenting cells (APCs) can take place over long distances. Our work has ... More
Inhibition of NF-?B-mediated signaling by the cyclin-dependent kinase inhibitor CR8 overcomes prosurvival stimuli to induce apoptosis in chronic lymphocytic leukemia cells.
AuthorsCosimo E, McCaig AM, Carter-Brzezinski LJ, Wheadon H, Leach MT, Le Ster K, Berthou C, Durieu E, Oumata N, Galons H, Meijer L, Michie AM,
JournalClin Cancer Res
PubMed ID23532892
Chronic lymphocytic leukemia (CLL) is currently incurable with standard chemotherapeutic agents, highlighting the need for novel therapies. Overcoming proliferative and cytoprotective signals generated within the microenvironment of lymphoid organs is essential for limiting CLL progression and ultimately developing a cure. We assessed the potency of cyclin-dependent kinase (CDK) inhibitor CR8, ... More
PKCa negatively regulates in vitro proplatelet formation and in vivo platelet production in mice.
AuthorsWilliams CM, Harper MT, Poole AW,
JournalPlatelets
PubMed ID23402219
Proplatelet formation is a part of the intricate process by which platelets are generated by their precursor cell, the megakaryocyte. The processes that drive megakaryocyte maturation and platelet production are however still not well understood. The protein kinase C (PKC) family of serine/threonine kinases has been demonstrated as an important ... More
CD26 inhibition enhances perfusion recovery in ApoE-/-mice.
AuthorsHaverslag RT, de Groot D, Grundmann S, Meder B, Goumans MJ, Pasterkamp G, Hoefer IE, de Kleijn DP,
JournalCurr Vasc Pharmacol
PubMed ID23391419
The adaptive growth of blood vessels is important to prevent tissue loss following arterial occlusion. Extravasation of monocytes is essential for this process. The peptidase CD26 targets SDF-1 alpha, a chemokine regulating monocyte trafficking. We hypothesized that blocking SDF-1 alpha inactivation, using a commercially available CD26 inhibitor, accelerates perfusion recovery ... More
The effect of Epstein-Barr virus Latent Membrane Protein 2 expression on the kinetics of early B cell infection.
AuthorsWasil LR, Tomaszewski MJ, Hoji A, Rowe DT,
JournalPLoS One
PubMed ID23308294
Infection of human B cells with wild-type Epstein-Barr virus (EBV) in vitro leads to activation and proliferation that result in efficient production of lymphoblastoid cell lines (LCLs). Latent Membrane Protein 2 (LMP2) is expressed early after infection and previous research has suggested a possible role in this process. Therefore, we ... More
Tumor necrosis factor receptor 1 associates with CD137 ligand and mediates its reverse signaling.
AuthorsMoh MC, Lorenzini PA, Gullo C, Schwarz H,
JournalFASEB J
PubMed ID23620528
Reverse signaling through CD137 ligand (CD137L) potently activates monocytes. However, the underlying mechanism is not well elucidated. This study provides evidence that tumor necrosis factor receptor 1 (TNFR1) acts as a coreceptor for CD137L and mediates CD137L signaling. CD137L colocalizes with TNFR1 on the plasma membrane and binds directly to ... More