CountBright™ Plus Absolute Counting Beads
CountBright™ Plus Absolute Counting Beads
Invitrogen™

CountBright™ Plus Absolute Counting Beads

CountBright Plus Absolute Counting Beads are a calibrated suspension of ∼4 μm beads that encapsulate dyes that are excitable acrossRead more
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Catalog NumberQuantity
C36995100 tests
Catalog number C36995
Price (USD)
369.00
Each
Add to cart
Quantity:
100 tests
Price (USD)
369.00
Each
Add to cart
CountBright Plus Absolute Counting Beads are a calibrated suspension of ∼4 μm beads that encapsulate dyes that are excitable across a wide range of UV-IR wavelengths and brightly fluoresce in the emission range of 385-860 nm. In instances where a flow cytometer is not designed to provide absolute cell count, the use of absolute counting beads can help to determine the count. Absolute cell count is defined as the concentration of cells in the original sample.

• Beads are on-scale with most cell types, including human whole lysed blood, for ease of use
• >95% singlets for improved accuracy
• Excited by UV-IR lasers for an expanded emission range (385–860 nm)
For Research Use Only. Not for use in diagnostic procedures.
Specifications
Diameter (Metric)4 μm
Quantity100 tests
Shipping ConditionRoom Temperature
Product LineCountBright™
Product TypeCell Counting Bead
Unit SizeEach
Contents & Storage
  • 5 mL beads in buffer
  • Store at 2°C to 8°C

Frequently asked questions (FAQs)

With the CountBright Plus Absolute Counting Beads/CountBright Absolute Counting Beads, instead of a tight cluster of beads on the scatter plot, I am seeing the beads cover a broad area. What is causing this?

Prior to taking an aliquot, make certain to thoroughly mix the beads. This may also be caused if the beads were ever frozen or the stock solution has any microbial contamination.

Find additional tips, troubleshooting help, and resources within our Cell Analysis Support Center.

Which buffers can I use with the CountBright Plus Absolute Counting Beads and CountBright Absolute Counting Beads?

You may use any physiological buffer or media in a pH range from pH 4 to 10. Avoid extremes of pH and high salt concentrations.

Find additional tips, troubleshooting help, and resources within our Cell Analysis Support Center.

How are CountBright Plus Absolute Counting Beads different from CountBright Absolute Counting Beads?

Here are the differences:

CountBright Plus Absolute Counting Beads:
Bead Size: 4 µm
Excitation Range: 385 - 810 nm
Emission Range: 385 - 860 nm

CountBright Absolute Counting Beads:
Bead Size: 7 µm
Excitation Range: 350 - 635 nm
Emission Range: 350 - 810 nm

Find additional tips, troubleshooting help, and resources within our Cell Analysis Support Center.