Carboxy-H2DCFDA (allgemeiner Indikator für oxidativen Stress)

Zitierungen und Referenzen (95)

Invitrogen™

Carboxy-H2DCFDA (allgemeiner Indikator für oxidativen Stress)

Katalognummer	Menge
C400	25 mg

Katalognummer C400

Preis (EUR)

445,65

Exklusiv online

474,00

Ersparnis 28,35 (6%)

Zum Warenkorb hinzufügen

Menge:

25 mg

Preis (EUR)

445,65

Exklusiv online

474,00

Ersparnis 28,35 (6%)

Zum Warenkorb hinzufügen

6-Carboxy-2',7'-Dichlordihydrofluorescein-Diacetat ist die chemisch reduzierte Acetylform von Fluorescein und wird als Indikator für reaktive Sauerstoffspezies (ROS) in Zellen verwendet. Dieses nicht fluoreszierende Molekül wird leicht in eine grün-fluoreszierende Form umgewandelt, wenn Acetatgruppen durch intrazelluläre Esterasen entfernt werden und innerhalb der Zelle Oxidation (durch die Aktivität von ROS) auftritt. Unser Angebot umfasst das carboxylierte H2DCFDA-Analogon (Carboxy-H2DCFDA, C400), das bei physiologischem pH zwei negative Ladungen hat, und sein Di(Acetoxymethylester) (C2938). Nach der Spaltung der Acetat- und Estergruppen durch intrazelluläre Esterasen und Oxidation bilden beide Analoga Carboxydichlorfluorescein (C368), mit zusätzlichen negativen Ladungen, die das Auslaufen aus der Zelle verhindern.

Spezifikationen des ROS-Indikators:
• Ex/Em: ∼492 – 495/517 – 527 nm
• Produkt ist luftempfindlich und sollte unter trockenem Argon oder Stickstoff gelagert werden
• Produkt kann zur Verwendung in DMSO, DMF oder Ethanol gelöst werden
• Membrangängiger Indikator (Zellladeprotokolle sind in den Literaturhinweisen erhältlich)
• Die Fluoreszenz kann durch Durchflusszytometer, Fluorometer, Mikrotiterplatten-Lesegeräte oder Fluoreszenzmikroskope mit Anregungsquellen und Filtern für Fluorescein überwacht werden

Weitere ROS-Indikatoren
Wir bieten ein Sortiment an Molecular Probes™ Produkten für die Herstellung reaktiver Sauerstoffspezies (ROS) an, einschließlich Singulett-Sauerstoff, Superoxid, Hydroxyl-Radikal und verschiedenen Peroxiden und Hydroperoxiden sowie für deren fluorometrischen Nachweis in Lösung. Weitere Informationen zu diesen Produkten finden Sie im Abschnitt Herstellung und Nachweis reaktiver Sauerstoffspezies – Abschnitt 18.2 im Molecular Probes™ Handbuch.

Für Forschungszwecke. Nicht für therapeutische oder diagnostische Zwecke bei Menschen oder Tieren vorgesehen.

Nur für Forschungszwecke. Nicht zur Verwendung bei diagnostischen Verfahren.

Specifications

Menge25 mg

ProdukttypROS Indicator

Unit SizeEach

Häufig gestellte Fragen (FAQ)

I want to assay cells for reactive oxygen species using carboxy-H2DCFDA, but I want to do so with a plate reader instead of microscope. Will it work?

It has been done. The problem is that plate readers are less sensitive than microscopes, with far less signal-to-background difference. It is worth trying, but first optimize concentrations and loading times with control cells, use a plate with little to no autofluorescence, and possibly optimize the gain setting in order to get the best signal possible. But don't expect the same sensitivity, even with optimization.

Find additional tips, troubleshooting help, and resources within our Cell Analysis Support Center.

I have GFP-transfected cells and need to label for reactive oxygen species. Can I use H2DCFDA?

This is not recommended as the two dyes overlap in the emission wavelength. There are other ROS reagents available in different wavelengths, such as CellROX Deep Red, which emits in the far-red range (665 nm), or dihydroethidium, which is emits in the visible red range (620 nm).

Find additional tips, troubleshooting help, and resources within our Cell Analysis Support Center.

I labeled my cell with CM-H2DCFDA for reactive oxygen detection, but upon illuminating the cell there is a significant increase in fluorescence in the control cells. Why?

If the cell is overloaded with dye, the high intracellular concentration of the dye may lead to dye-dye quenching. Upon illumination, photobleaching will occur, which will reduce the dye-dye quenching and actually increase the fluorescence (for a while, but then it will start decreasing). To solve the problem, reduce the concentration and incubation time, and try a range of incubation times and concentrations.

Find additional tips, troubleshooting help, and resources within our Cell Analysis Support Center.

I need a formaldehyde-fixable reactive oxygen species detection assay. Is H2 DCFDA fixable?

H₂DCFDA and similar derivatives are not fixable. The same goes for dihydroethidium and dihydrorhodamine. However, CellROX Deep Red and CellROX Green are retained for a limited time upon fixation with formaldehyde. CellROX Green may be retained upon subsequent Triton X-100 permeabilization. Avoid the use of any acetone or alcohol-based fixatives or fixatives that include alcohol, such as formalin.

Find additional tips, troubleshooting help, and resources within our Cell Analysis Support Center.

Zitierungen und Referenzen (95)

Zitierungen und Referenzen

Abstract

N-acetylcysteine augments the cellular redox changes and cytotoxic activity of internalized mycobacterium bovis in human bladder cancer cells.

Authors:Pook SH, Esuvaranathan K, Mahendran R

Journal:J Urol

PubMed ID:12131368

PURPOSE: We determined whether changes in cellular reactive oxygen species correlated with mycobacteria internalization and bladder cancer cell death. MATERIALS AND METHODS: Reactive oxygen species and thiols in RT112 and MGH bladder cancer cells were determined using the fluorescence probes 5-(and 6)-carboxy-2', 7' dichlorodihydrofluorescein diacetate and monobromobimane. Superoxide and nitrite ... More

The sulphonylurea glibenclamide inhibits multidrug resistance protein (MRP1) activity in human lung cancer cells.

Authors:Payen L, Delugin L, Courtois A, Trinquart Y, Guillouzo A, Fardel O

Journal:Br J Pharmacol

PubMed ID:11159731

'1. Glibenclamide, a sulphonylurea widely used for the treatment of non-insulin-dependent diabetes mellitus, has been shown to inhibit the activities of various ATP-binding cassette (ABC) transporters. In the present study, its effects towards multidrug resistance protein 1 (MRP1), an ABC efflux pump conferring multidrug resistance and handling organic anions, were ... More

Characterization of Vta1p, a class E Vps protein in Saccharomyces cerevisiae.

Authors:Shiflett SL, Ward DM, Huynh D, Vaughn MB, Simmons JC, Kaplan J

Journal:J Biol Chem

PubMed ID:14701806

'We identified VTA1 in a screen for mutations that result in altered vacuole morphology. Deletion of VTA1 resulted in delayed trafficking of the lipophilic dye FM4-64 to the vacuole and altered vacuolar morphology when cells were exposed to the dye 5-(and 6)-carboxy-2'',7''-dichlorofluorescein diacetate (CDCFDA). Deletion of class E vacuolar protein ... More

Mitochondrial localization of reactive oxygen species by dihydrofluorescein probes.

Authors:Diaz G, Liu S, Isola R, Diana A, Falchi AM

Journal:Histochem Cell Biol

PubMed ID:14574587

'Mitochondria are the main source of reactive oxygen species (ROS). The aim of this work was to verify the ROS generation in situ in HeLa cells exposed to prooxidants and antioxidants (menadione, tert-butyl hydroperoxide, antimycin A, vitamin E, N-acetyl-L-cysteine, and butylated hydroxytoluene) using the ROS-sensitive probes 6-carboxy-2'',7''-dichlorodihydrofluorescein diacetate di-acetomethyl ester ... More

The use of fluorescent probes to assess oxidative processes in isolated-perfused rat heart tissue.

Authors:Kehrer JP, Paraidathathu T

Journal:Free Radic Res Commun

PubMed ID:1505782

'The formation of reactive oxygen species (ROS) in intact heart tissue has been assessed by direct ESR measurements, and indirectly by the formation of characteristic tissue products and the protective effects of various antioxidants. The development of lipid soluble esters of compounds which can be trapped intracellularly after hydrolysis, and ... More

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