One Shot® TOP10 Electrocomp™ E. coli

Catalog number: C404050

Invitrogen™  Related applications: Transformation

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Description

One Shot® TOP10 Electrocomp™ E. coli are provided at a transformation efficiency of 1 x 1010 cfu/ µg supercoiled DNA and are ideal for high-efficiency cloning and plasmid propagation. They allow stable replication of high copy number plasmids. The genotype of TOP10 cells is similar to that of the DH10B™ strain, and offers the following features:

hsdR for efficient transformation of unmethylated DNA from PCR amplifications
mcrA for efficient transformation of methylated DNA from genomic preparations
lacZΔM15 for blue/white color screening of recombinant clones
endA1 for cleaner preparations of DNA—get better results in downstream applications due to the elimination of nonspecific digestion by Endonuclease I
recA1 for reduced occurrence of nonspecific recombination in cloned DNA

Kit options
One Shot® TOP10 Kits are available with either chemically competent or electrocompetent E. coli to fit your specific transformation needs. TOP10 E. coli are also available in the high-throughput MultiShot™ format.
For Research Use Only. Not for use in diagnostic procedures.

Specifications

Competent Cell Type: Electrocompetent
Species: E. coli
Format: One Shot
Transformation Efficiency: > 1x10^10
Bacterial or Yeast Strain: TOP10
Blue-White Screening: Yes
Cloning Methylated DNA: Yes
Cloning Unstable DNA: Not Suitable for cloning unstable DNA
F' Episome (to make ssDNA): Lacks F' Episome
High Throughput Compatibility: Not High Throughput-Compatible (Manual)
Improves Plasmid Quality: Yes
Plasmid Type: May be used for plasmids > 20 kb
Preparing Unmethylated DNA: Not Suitable for preparing unmethylated DNA
Product Size: 10 reactions
Propagating ccdB Vectors: Not for ccdB vector propagation
Reduces Recombination: Yes
T1 Phage - Resistant (tonA): No
Shipping Condition: Dry Ice

Contents & storage

Store in ultra-cold freezer (-68 to -85°C).

Documents

Manuals & protocols

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