Invitrogen™

One Shot™ TOP10/P3 Chemically Competent E. coli

Catalog number: C505003
Invitrogen™

One Shot™ TOP10/P3 Chemically Competent E. coli

Catalog number: C505003
Catalog Number
C505003
also known as C5050-03
Unit Size
21 x 50 µL/tube
Price (USD)
Price: 532.00
Your price:
Availability
-
Quantity
Catalog NumberUnit SizePrice (USD)AvailabilityQuantity
C505003
also known as C5050-03
21 x 50 µL/tube
Price: 532.00
Your price:
-
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One Shot TOP10/P3 Chemically Competent E. coli cells are designed for vector transformation encoding the synthetic supF gene (tyrosine tRNA suppressor) and allow high-quality pCDM8, pcDNA1.1 plasmid, or any other supF-containing vector preparation.

One Shot TOP10/P3 strain features
• P3 plasmid for selection of supF-containing plasmids
• Mutated endA1 gene for increased quality of plasmid DNA preparations
recA1 for reduced occurrence of non-specific recombination in cloned DNA
lacZΔM15 for blue/white color screening of recombinant clones

P3 Plasmid
The P3 plasmid is a low-copy-number, 60 kb plasmid that carries kanamycin, tetracycline and ampicillin resistance markers. The kanamycin gene is fully active and is used to select for cells carrying P3. Tetracycline and ampicillin genes carry amber mutations which render the genes inactive during normal growth and replication of the bacteria. Upon transformation of a vector carrying the suppressor F gene, the amber mutations in the tetracycline and ampicillin genes on the P3 plasmid are suppressed and the E. coli are resistant to these antibiotics.

Genotype
F- mcrA Δ(mrr-hsdRMS-mcrBC) φ80lacZΔM15 ΔlacX74 recA1 araD139 Δ(ara-leu)7697 galU galK rpsL endA1 nupG P3: KanR AmpR (am) TetR (am) 

Find the strain and format that you need
TOP10 is offered in chemically competent and electrocompetent cell formats.
Choose MultiShot competent cells for high-throughput cloning applications.
Email us for more information about custom formats.

For Research Use Only. Not for use in diagnostic procedures.

Specifications

Product Type
Chemically Competent Cells
Contains F' Episome
No
Improves Plasmid Quality
Yes (endA1)
Cloning Methylated DNA
Yes (mcrA)
Transformation Efficiency Level
Medium Efficiency (1 x 108 to 1 x 109 cfu/μg)
Antibiotic Resistance Bacterial
Yes (Streptomycin, Kanamycin, Ampicillin, Tetracycline)
Cloning Unstable DNA
No
Blue/White Screening
Yes (lacZΔM15)
High-throughput Compatibility
Low
Preparing Unmethylated DNA
No
Reduces Recombination
Yes (recA1)
Shipping Condition
Dry Ice
T1 Phage - Resistant (tonA)
No
Species
E. coli (K12)
Format
Tube
Product Line
One Shot™
Quantity
21 x 50 μL

Contents & Storage

• One Shot TOP10/P3 Chemically Competent E. coli (21 x 50 μL)
Store Competent Cells at –80°C.

• pUC19 Plasmid (50 μL)
Store pUC19 plasmid at –20°C.

S.O.C. Medium (6 mL)
Store S.O.C. Medium at 4°C or at room temperature

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