Invitrogen™

One Shot™ TOP10/P3 Chemically Competent E. coli

Catalog number:  C505003

Related applications:

Cloning

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Description

TOP10/P3 E. coliallow high-quality plasmid preparation of pCDM8, pcDNA™1.1, or any other supF-containing vector. The strain features:

• P3 plasmid for selection of supF-containing plasmids
• Mutated endA1 gene for increased quality of plasmid DNA preparations
For Research Use Only. Not for use in diagnostic procedures.

Specifications

Bacterial or Yeast Strain: TOP10/P3
Blue/White Screening: Yes
Cloning Methylated DNA: Yes
Cloning Unstable DNA: Not Suitable for cloning unstable DNA
Competent Cell Type: Chemically Competent
F'episome (to make ssDNA): Lacks F' Episome
High-throughput Compatibility: Not High-throughput Compatible (Manual)
Preparing Unmethylated DNA: Not Suitable for preparing unmethylated DNA
Product Line: One Shot™
Propagating ccdB Vectors: Not for ccdB vector propagation
Reduces Recombination: Yes
T1 Phage - Resistant (tonA): No
Transformation Efficiency: > 5x10^6
Transformation Efficiency Level: Medium Efficiency (10^8-10^9 cfu⁄µg)
Format: One Shot
Improves Plasmid Quality: Yes
Species: E. coli