One Shot® TOP10/P3 Chemically Competent E. coli

Catalog number: C505003

Invitrogen™  Related applications: Transformation

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Description

TOP10/P3 E. coliallow high-quality plasmid preparation of pCDM8, pcDNA™1.1, or any other supF-containing vector. The strain features:

• P3 plasmid for selection of supF-containing plasmids
• Mutated endA1 gene for increased quality of plasmid DNA preparations
For Research Use Only. Not for use in diagnostic procedures.

Specifications

Competent Cell Type: Chemically Competent
Species: E. coli
Format: One Shot
Transformation Efficiency: > 5x10^6
Bacterial or Yeast Strain: TOP10/P3
Blue-White Screening: Yes
Cloning Methylated DNA: Yes
Cloning Unstable DNA: Not Suitable for cloning unstable DNA
F' Episome (to make ssDNA): Lacks F' Episome
High Throughput Compatibility: Not High Throughput-Compatible (Manual)
Improves Plasmid Quality: Yes
Preparing Unmethylated DNA: Not Suitable for preparing unmethylated DNA
Product Size: 20 x 50 µL
Propagating ccdB Vectors: Not for ccdB vector propagation
Reduces Recombination: Yes
T1 Phage - Resistant (tonA): No

Documents

Manuals & protocols