Invitrogen™

One Shot™ BL21(DE3) Chemically Competent E. coli

Catalog number: C600003
Invitrogen™

One Shot™ BL21(DE3) Chemically Competent E. coli

Catalog number: C600003
Catalog Number
C600003
also known as C6000-03
Unit Size
21 x 50 µL/tube
Price (USD)
317.00
Your Price:
Availability
***
Quantity
Catalog NumberUnit SizePrice (USD)AvailabilityQuantity
C600003
also known as C6000-03
21 x 50 µL/tube
317.00
Your Price:
***
Product Overview
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Additional Information
Recommendations
One Shot® BL21(DE3) Chemically Competent E. coli are ideal for use with bacteriophage T7 promoter-based expression systems (e.g., pRSET, pCR™T7, and pET). BL21(DE3) cells carry the lambda DE3 lysogen. Recombinant proteins that are nontoxic to E. coli are generally expressed at higher levels in BL21(DE3) cells than in BL21(DE3)pLysS or BL21(DE3)pLysE. However, the basal expression levels of heterologous genes are significantly higher in BL21(DE3) than in BL21(DE3)pLysS or BL21(DE3)pLysE.

About BL21 strains
BL21 strains are descended from the E. coli B strain and have been specifically constructed for high-level expression of recombinant proteins. These strains have two important attributes that make them ideal for protein expression: key genetic markers and inducibility of protein expression. The most important genetic markers help recombinant RNA and/or protein accumulate to high levels without degradation. Inducibility helps to minimize the toxic effects of some recombinant proteins.
For Research Use Only. Not for use in diagnostic procedures.

Specifications

Bacterial or Yeast Strain
BL21(DE3)
Blue/White Screening
No
Cloning Methylated DNA
No
Competent Cell Type
Chemically Competent
F'episome (to make ssDNA)
Lacks F' Episome
High-throughput Compatibility
Not High-throughput Compatible (Manual)
Propagating ccdB Vectors
Not for ccdB vector propagation
Reduces Recombination
No
T1 Phage - Resistant (tonA)
No
Transformation Efficiency
> 1x10^8
Transformation Efficiency Level
Medium Efficiency (10^8-10^9 cfu⁄µg)
Format
One Shot
Product Line
One Shot™
Product Type
Competent Cells
Quantity
20 x 50 µL
Species
E. coli
Improves Plasmid Quality
No

Contents & Storage

Contains:
• One Shot™ BL21(DE3) E.coli: 20 vials, 50 µl each (total of 1 ml)
• pUC19 DNA (10 pg/ul): 1 vial, 50 µl
• SOC Medium: 1 bottle, 6 ml

Store Competent Cells at -80°C. Store pUC19 DNA at -20°C. Store SOC Medium at 4°C or at room temperature.

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Citations & References