BL21(DE3)pLysS cells are ideal for use with T7 promoter-based expression systems (e.g., pRSET, pCR™T7, and pET). BL21(DE3)pLysS E. coli carry both the DE3 lysogen and the plasmid pLysS. pLysS constitutively expresses low levels of T7 lysozyme, which reduces basal expression of recombinant genes by inhibiting basal levels of T7 RNA polymerase.
For Research Use Only. Not for use in diagnostic procedures.