Invitrogen™

One Shot™ BL21(DE3)pLysS Chemically Competent E. coli

Catalog number: C606003
Invitrogen™

One Shot™ BL21(DE3)pLysS Chemically Competent E. coli

Catalog number: C606003
Catalog Number
C606003
also known as C6060-03
Unit Size
21 x 50 µL/tube
Price (USD)
349.00
Your Price:
Availability
***
Quantity
Catalog Number
C606010
also known as C6060-10
Unit Size
11 x 50 µL/tube
Price (USD)
210.00
Your Price:
Availability
***
Quantity
Catalog NumberUnit SizePrice (USD)AvailabilityQuantity
C606003
also known as C6060-03
21 x 50 µL/tube
349.00
Your Price:
***
C606010
also known as C6060-10
11 x 50 µL/tube
210.00
Your Price:
***
Product Overview
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Additional Information
Recommendations
BL21(DE3)pLysS cells are ideal for use with T7 promoter-based expression systems (e.g., pRSET, pCR™T7, and pET). BL21(DE3)pLysS E. coli carry both the DE3 lysogen and the plasmid pLysS. pLysS constitutively expresses low levels of T7 lysozyme, which reduces basal expression of recombinant genes by inhibiting basal levels of T7 RNA polymerase.
For Research Use Only. Not for use in diagnostic procedures.

Specifications

Bacterial or Yeast Strain
BL21(DE3), BL21(DE3)pLysS
Blue/White Screening
No
Cloning Methylated DNA
No
Competent Cell Type
Chemically Competent
F'episome (to make ssDNA)
Lacks F' Episome
High-throughput Compatibility
Not High-throughput Compatible (Manual)
Propagating ccdB Vectors
Not for ccdB vector propagation
Reduces Recombination
No
T1 Phage - Resistant (tonA)
No
Transformation Efficiency
> 1x10^8
Transformation Efficiency Level
Medium Efficiency (10^8-10^9 cfu⁄µg)
Format
One Shot
Product Line
One Shot™
Product Type
Competent Cells
Quantity
20 x 50μL
Species
E. coli
Improves Plasmid Quality
No

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Citations & References