Invitrogen™

BL21-AI™ One Shot™ Chemically Competent E. coli

Catalog number: C607003
Invitrogen™

BL21-AI™ One Shot™ Chemically Competent E. coli

Catalog number: C607003
Catalog Number
C607003
also known as C6070-03
Unit Size
21 x 50 µL/tube
Price (USD)
406.00
Your Price:
Availability
***
Quantity
Catalog NumberUnit SizePrice (USD)AvailabilityQuantity
C607003
also known as C6070-03
21 x 50 µL/tube
406.00
Your Price:
***
Product Overview
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One Shot® BL21-AI™ E. coli are chemically competent cells designed for applications that require tight regulation and strong expression of toxic proteins from any T7 promoter-based expression systems. One Shot® BL21-AI™ Chemically Competent cells have a transformation efficiency of >1×108 cfu/ µg plasmid DNA.
• Ideal for inducing expression of toxic protein
• Gene expression is regulated by the addition of L-arabinose to culture
• Suitable for use with any T7 promoter-based plasmids
• Supplied in a convenient One Shot® format

Excellent Expression of Toxic Recombinant Proteins
One Shot® BL21-AI™ E. coli contains a chromosomal insertion of the gene encoding T7 RNA polymerase (T7RNAP) into the araB locus of the araBAD operon, placing regulation of T7 RNAP under the control of the arabinose-inducible araBAD promoter (1). Therefore, this strain is especially useful for the expression of genes that may be toxic to other BL21 strains where basal expression of T7 RNAP is leaky. The BL21-AI™ strain does not contain the Ion protease and is deficient in the outer membrane protease, OmpT. This genotype reduces degradation of heterologous proteins expressed in this strain. Finally, the yield of recombinant protein obtained from BL21-AI™ is generally similar to that obtained from other BL21 strains.

Genotype:
F-ompT hsdSB (rB- mB-) gal dcm araB::T7RNAP-tetA

Easy-to-use, Single Tube Format
The single tube, single use, format allows for all transformation steps to take place in the same tube, thereby helping to save time and prevent contamination.

Find the Strain and Format That You Need
We also offer many other different strains and formats of chemically competent cells and electrocompetent cells to help meet your specific transformation needs. For expression of non-toxic recombinant proteins, choose our One Shot® BL21 Star™ (DE3) chemically competent cells or One Shot® BL21 Star™ (DE3) pLysS chemically competent cells.

For Research Use Only. Not intended for animal or human diagnostic or therapeutic use.
For Research Use Only. Not for use in diagnostic procedures.

Specifications

Bacterial or Yeast Strain
BL21-AI™
Blue/White Screening
No
Cloning Methylated DNA
No
Competent Cell Type
Chemically Competent
F'episome (to make ssDNA)
Lacks F' Episome
High-throughput Compatibility
Not High-throughput Compatible (Manual)
Propagating ccdB Vectors
Not for ccdB vector propagation
Reduces Recombination
No
T1 Phage - Resistant (tonA)
No
Transformation Efficiency
> 1x10^8
Transformation Efficiency Level
Medium Efficiency (10^8-10^9 cfu⁄µg)
Format
One Shot
Product Line
One Shot™
Product Type
Competent Cells
Quantity
20 x 50 µL
Species
E. coli
Improves Plasmid Quality
No

Contents & Storage

BL21-AI™ Chemically Competent E. coli are provided in single-use, 50-µl aliquots, with S.O.C. medium and supercoiled control plasmid. Store competent cells at -80°C. All components are guaranteed stable for 6 months when properly stored.

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