Cross-resistance to antifolates in multidrug resistant cell lines with P-glycoprotein or multidrug resistance protein expression.
Authorsvan Triest B, Pinedo HM, Telleman F, van der Wilt CL, Jansen G, Peters GJ
JournalBiochem Pharmacol
PubMed ID9256160
'Resistance to some (lipophilic) antifolates has been associated with P-glycoprotein (P-gp)-mediated multidrug resistance (MDR). A possible relationship with non-P-gp MDR has not been established. We studied resistance to antifolates in SW-1573 human lung carcinoma cells, a P-gp overexpressing variant SW-1573/2R160 and a multidrug resistance protein (MRP) overexpressing variant SW-1573/2R120. In ... More
Down-regulation of topoisomerase II by camptothecin does not prevent additive activity of the topoisomerase II inhibitor etoposide in vitro.
AuthorsStahl M, Kasimir-Bauer S, Harstrick A
JournalAnticancer Drugs
PubMed ID9311443
'Topoisomerases (Topo) I and II are cellular enzymes that catalyze the relaxation of topologically strained DNA and that are involved in a number of DNA-related processes. Their complete inhibition by Topo I and II inhibitors gives promise for improvements in the treatment of malignant diseases. However, preclinical studies showed down-regulation ... More
Evidence for uptake of vital dye by activated rat peritoneal mast cells: an in vitro imaging study.
AuthorsWei JY, Go VL, Taché Y, Kruger L
JournalNeuroimage
PubMed ID9343581
'Uptake of material from surrounding medium by activated rat peritoneal mast cells (PMCs) was studied using in vitro peritoneal eluate cells, the vital fluorescent dye sulforhodamine B (SFRM-B), secretagogue compound 48/80, and an imaging technique. PMCs, which undergo different states of degranulation, are shown to possess the ability to take ... More
Growth inhibition of human glioma cells induced by 8-chloroadenosine, an active metabolite of 8-chloro cyclic adenosine 3':5'-monophosphate.
AuthorsLangeveld CH, Jongenelen CA, Heimans JJ, Stoof JC
JournalCancer Res
PubMed ID1617676
'8-Chloroadenosine 3'':5''-monophosphate (8ClcAMP) inhibits the growth of human glioma cell lines at much lower concentrations than more commonly used cyclic AMP analogues, without inducing morphological differentiation. The mechanism by which 8ClcAMP exerts this effect is not fully understood. We examined whether the growth-inhibitory effect of this compound is due to ... More
Localization, physiology, and modulation of a molluskan dopaminergic synapse.
AuthorsMagoski NS, Bulloch AG
JournalJ Neurobiol
PubMed ID9298763
'We investigated the location, physiology, and modulation of an identified synapse from the central nervous system (CNS) of the mollusk Lymnaea stagnalis. Specifically, the excitatory synapse from interneuron right pedal dorsal one (RPeD1) to neurons visceral dorsal two and three (VD2/3) was examined. The gross and fine morphology of these ... More
Importance of pharmacodynamics in the in vitro antiproliferative activity of the antifolates methotrexate and 10-ethyl-10-deazaaminopterin against human head and neck squamous cell carcinoma.
AuthorsBraakhuis BJ, Jansen G, Noordhuis P, Kegel A, Peters GJ
JournalBiochem Pharmacol
PubMed ID7506025
'The pharmacodynamic profiles of methotrexate (MTX) and 10-ethyl-10-deazaaminopterin (10-EdAM) were determined in three head and neck squamous cell carcinoma (HNSCC) cell lines. Cell growth inhibition was tested using a semi-automated 96-well based proliferation assay, the sulforhodamine B (SRB) assay. Drug concentrations ranged from 10(-5) to 10(-9) M, with exposure periods ... More
Convergent force fields organized in the frog's spinal cord.
AuthorsGiszter SF, Mussa-Ivaldi FA, Bizzi E
JournalJ Neurosci
PubMed ID8426224
'Microstimulation of the gray matter of the frog''s spinal cord was used to elicit motor responses. Force responses were recorded with the frog''s ankle clamped while EMG activity was monitored. The collections of force patterns elicited at different leg configurations were summarized as force fields. These force fields showed convergence ... More
Fusion pore dynamics and insulin granule exocytosis in the pancreatic islet.
AuthorsTakahashi N, Kishimoto T, Nemoto T, Kadowaki T, Kasai H
JournalScience
PubMed ID12193788
Insulin secretion from intact mouse pancreatic islets was investigated with two-photon excitation imaging. Insulin granule exocytosis occurred mainly toward the interstitial space, away from blood vessels. The fusion pore was unusually stable with a lifetime of 1.8 seconds. Opening of the 1.4-nanometer-diameter pore was preceded by unrestricted lateral diffusion of ... More
Restricted movement of lipid and aqueous dyes through pores formed by influenza hemagglutinin during cell fusion.
AuthorsZimmerberg J, Blumenthal R, Sarkar DP, Curran M, Morris SJ
JournalJ Cell Biol
PubMed ID7806567
The fusion of cells by influenza hemagglutinin (HA) is the best characterized example of protein-mediated membrane fusion. In simultaneous measurements of pairs of assays for fusion, we determined the order of detectable events during fusion. Fusion pore formation in HA-triggered cell-cell fusion was first detected by changes in cell membrane ... More
Growth, morphology and chemosensitivity studies on postconfluent cells cultured in 'V'-bottomed microtiter plates.
This study assessed the growth pattern, cellular organisation and chemosensitivity of established human tumour cell lines growing as postconfluent cultures in 'V'-bottomed, 96-well microtiter plates. Cross-sections of the colon (HT29, SW620, SW1116), ovarian (A2780) and head and neck (UM-SCC-22B) carcinoma microcultures allowed in situ evaluation of the cellular organisation in ... More
Feasibility of a high-flux anticancer drug screen using a diverse panel of cultured human tumor cell lines.
AuthorsMonks A, Scudiero D, Skehan P, Shoemaker R, Paull K, Vistica D, Hose C, Langley J, Cronise P, Vaigro-Wolff A
JournalJ Natl Cancer Inst
PubMed ID2041050
We describe here the development and implementation of a pilot-scale, in vitro, anticancer drug screen utilizing a panel of 60 human tumor cell lines organized into subpanels representing leukemia, melanoma, and cancers of the lung, colon, kidney, ovary, and central nervous system. The ultimate goal of this disease-oriented screen is ... More
Anti-invasive activity of alkaloids and polyphenolics in vitro.
AuthorsParmar VS, Bracke ME, Philippe J, Wengel J, Jain SC, Olsen CE, Bisht KS, Sharma NK, Courtens A, Sharma SK, Vennekens K, Van Marck V, Singh SK, Kumar N, Kumar A, Malhotra S, Kumar R, Rajwanshi VK, Jain R, Mareel MM
JournalBioorg Med Chem
PubMed ID9313866
Invasiveness, the ability of certain tumour cells to migrate beyond their natural tissue boundaries, often leads to metastasis, and usually determines the fatal outcome of cancer. The need for anti-invasive agents has led us to search for possibly active compounds among alkaloids and polyphenolics. One hundred compounds were screened in ... More
Antiproliferative effect of nonsteroidal antiinflammatory drugs against human colon cancer cells.
AuthorsHixson LJ, Alberts DS, Krutzsch M, Einsphar J, Brendel K, Gross PH, Paranka NS, Baier M, Emerson S, Pamukcu R
JournalCancer Epidemiol Biomarkers Prev
PubMed ID7920212
Several lines of evidence suggest that nonsteroidal antiinflammatory drugs may be effective in preventing colorectal cancer. These include animal experiments, case-control studies, and clinical experience with sulindac in promoting the regression of adenomatous colon polyps in adenomatous polyposis coli. We determined the antiproliferative activity of various nonsteroidal antiinflammatory drugs, including ... More
Antiproliferative activity of the topoisomerase I inhibitors topotecan and camptothecin, on sub- and postconfluent tumor cell cultures.
AuthorsPizao PE, Smitskamp-Wilms E, Van Ark-Otte J, Beijnen JH, Peters GJ, Pinedo HM, Giaccone G
JournalBiochem Pharmacol
PubMed ID7945408
We have assessed the antiproliferative effects of a 24-hr exposure to the topoisomerase I inhibitors, topotecan and camptothecin, on two colon and one ovarian human tumor cell lines, cultured as subconfluent and as multilayered postconfluent cultures. Chemosensitivity was measured by the sulforhodamine B assay. In general, postconfluent cultures were less ... More
Rapid sizing of short tandem repeat alleles using capillary array electrophoresis and energy-transfer fluorescent primers.
AuthorsWang Y, Ju J, Carpenter BA, Atherton JM, Sensabaugh GF, Mathies RA
JournalAnal Chem
PubMed ID7537476
Genetic typing of the short tandem repeat (STR) polymorphism HUMTHO1 has been performed using capillary array electrophoresis and energy-transfer fluorescent dye-labeled polymerase chain reaction primers. Target alleles were amplified by use of primers labeled with one fluorescein at the 5' end and another fluorescein at the position of the 15th ... More
Growth and chemotherapeutic response of cells in a hollow-fiber in vitro solid tumor model.
BACKGROUND: Cancer treatments that appear promising in tissue culture are often less effective in solid tumors, in part because of the proliferative and microenvironmental heterogeneity that develops in these tumors as they grow. Heterogeneous tumor models are thus needed for drug screening. PURPOSE: Our goal was to develop and test ... More
Evidence that zymogen granules are not a physiologically relevant calcium pool. Defining the distribution of inositol 1,4,5-trisphosphate receptors in pancreatic acinar cells.
AuthorsYule DI, Ernst SA, Ohnishi H, Wojcikiewicz RJ
JournalJ Biol Chem
PubMed ID9083036
A key event leading to exocytosis of pancreatic acinar cell zymogen granules is the inositol 1,4,5-trisphosphate (InsP3)-mediated release of Ca2+ from intracellular stores. Studies using digital imaging microscopy and laser-scanning confocal microscopy have indicated that the initial release of Ca2+ is localized to the apical region of the acinar cell, ... More
Establishment and characterization of an in vitro model of acquired resistance to cisplatin in a human testicular nonseminomatous germ cell line.
AuthorsKelland LR, Mistry P, Abel G, Freidlos F, Loh SY, Roberts JJ, Harrap KR
JournalCancer Res
PubMed ID1312897
Clinically, human testicular nonseminomatous germ cell tumors exhibit remarkable sensitivity to platinum-based chemotherapy. To define better the mechanistic basis for this unusual sensitivity, the biochemical determinants of platinum-induced cytotoxicity have been investigated in a human testicular tumor cell line (GCT27) established from a previously untreated patient and in an in ... More
Enhanced potency of daunorubicin against multidrug resistant subline KB-ChR-8-5-11 by a pulsed magnetic field.
AuthorsLiang Y, Hannan CJ, Chang BK, Schoenlein PV
JournalAnticancer Res
PubMed ID9216668
Tumor cell resistance to many unrelated anticancer drugs is a major obstacle during cancer chemotherapy. One mechanism of drug resistance is thought to be due to the efflux of anticancer drugs caused by P-glycoprotein. In recent years, magnetic fields have been found to enhance the potency of anticancer drugs, with ... More
High-resolution liquid chromatography of fluorescent dye-labeled nucleic acids.
Using 100 mM of triethylammonium acetate as ion-pairing reagent, phosphodiester oligonucleotides labeled fluorescently at their 5' terminus could be separated successfully on alkylated nonporous 2.3-microns poly(styrene-divinylbenzene) particles by means of high-resolution liquid chromatography. Applying excitation wavelengths of 490, 520, 550, and 575 nm, respectively, optimum sensitivity was achieved for the ... More
Human ovarian-carcinoma cell lines and companion xenografts: a disease-oriented approach to new platinum anticancer drug discovery.
AuthorsKelland LR, Jones M, Abel G, Valenti M, Gwynne J, Harrap KR
JournalCancer Chemother Pharmacol
PubMed ID1586979
A disease-oriented approach to the discovery of novel platinum anticancer drugs has been established through the setting up of parallel human ovarian-carcinoma cell lines and xenografts. The correlation between in vitro and in vivo antitumour activity was determined for four reference platinum agents (cisplatin, carboplatin, iproplatin and tetraplatin) in eight ... More
Modulation of P-glycoprotein activity by estramustine is limited by binding to plasma proteins.
BACKGROUND. Estramustine previously has been shown to interact with P-glycoprotein and to restore intracellular accumulation of vinblastine and paclitaxel in cells overexpressing this drug transporter. However, the ability of estramustine to potentiate the cytotoxicities of several drugs was less than that expected. To resolve this apparent discordance, the authors examined ... More
Combined action of paclitaxel and cisplatin against wildtype and resistant human ovarian carcinoma cells.
AuthorsLevasseur LM, Greco WR, Rustum YM, Slocum HK
JournalCancer Chemother Pharmacol
PubMed ID9332464
PURPOSE: The combination of paclitaxel (PTX) and cisplatin (DDP) shows good clinical efficacy against ovarian cancer. In order to examine the potential cellular basis for this, and provide leads as to how to optimize the combination, we examined the role of sequence of exposure to PTX and DDP on cell ... More