MC1061/P3 Chemically Competent E. coli

Catalog number:  C66303

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MC1061/P3 Chemically Competent E. coli are used for highly efficient transformation of vectors that require the P3 episome for selection and maintenance (i.e., pCDM8, pcDNA™1.1, or any other supF-containing vector).

• 5 x 300 μL competent MC1061/P3 E. coli
• 50 µL pUC19 plasmid DNA (10 pg/μL in 5 mM Tris-HCl, 0.5 mM EDTA, pH 8)
• 15 mL SOC medium

For Research Use Only. Not for use in diagnostic procedures.


Competent Cell Type: Chemically Competent
Species: E. coli
Format: Tube(s)
Transformation Efficiency: > 1x10^8
Bacterial or Yeast Strain: MC1061/P3
Blue-White Screening: No
Cloning Methylated DNA: Yes
Cloning Unstable DNA: Not Suitable for cloning unstable DNA
F' Episome (to make ssDNA): Lacks F' Episome
High Throughput Compatibility: Not High Throughput-Compatible (Manual)
Improves Plasmid Quality: No
Preparing Unmethylated DNA: Not Suitable for preparing unmethylated DNA
Product Size: 1.5 mL
Propagating ccdB Vectors: Not for ccdB vector propagation
Reduces Recombination: No
T1 Phage - Resistant (tonA): No


Manuals & protocols