Invitrogen™

MC1061/P3 Chemically Competent E. coli

Catalog number:  C66303

Related applications:

Transformation

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Description

MC1061/P3 Chemically Competent E. coli are used for highly efficient transformation of vectors that require the P3 episome for selection and maintenance (i.e., pCDM8, pcDNA™1.1, or any other supF-containing vector).

Contents
• 5 x 300 μL competent MC1061/P3 E. coli
• 50 µL pUC19 plasmid DNA (10 pg/μL in 5 mM Tris-HCl, 0.5 mM EDTA, pH 8)
• 15 mL SOC medium

For Research Use Only. Not for use in diagnostic procedures.

Specifications

Bacterial or Yeast Strain: MC1061/P3
Blue/White Screening: No
Cloning Methylated DNA: Yes
Cloning Unstable DNA: Not Suitable for cloning unstable DNA
Competent Cell Type: Chemically Competent
F'episome (to make ssDNA): Lacks F' Episome
High-throughput Compatibility: Not High-throughput Compatible (Manual)
Preparing Unmethylated DNA: Not Suitable for preparing unmethylated DNA
Propagating ccdB Vectors: Not for ccdB vector propagation
Reduces Recombination: No
T1 Phage - Resistant (tonA): No
Transformation Efficiency: > 1x10^8
Transformation Efficiency Level: Medium Efficiency (10^8-10^9 cfu⁄µg)
Format: Tube(s)
Improves Plasmid Quality: No
Species: E. coli