Invitrogen™

TOP10F´ Electrocomp™ Kit

Catalog number: C66511
Invitrogen™

TOP10F´ Electrocomp™ Kit

Catalog number: C66511
Catalog Number
C66511
also known as C665-11
Unit Size
10 x 100 µL
Price (USD)
Price: 538.00
Your price:
Availability
-
Quantity
Catalog NumberUnit SizePrice (USD)AvailabilityQuantity
C66511
also known as C665-11
10 x 100 µL
Price: 538.00
Your price:
-
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TOP10F´ Electrocomp Kit E. coli cells are identical to TOP10 cells, with the addition of an F´ episome. The F´ episome carries the tetracycline resistance gene and allows isolation of single-stranded DNA (ssDNA) from vectors that have an f1 origin of replication. In addition, the F´ episome carries the lacIq repressor for inducible expression from trc, tac, and lac promoters using IPTG.

TOP10F´ competent cells are transformed via electroporation, thus achieving higher transformation efficiencies than chemically competent cells and may be used in more challenging applications, including cloning a low amount of DNA or cDNA and gDNA library construction. These cells allow stable replication of high copy number plasmids and are provided at a transformation efficiency of >1 x 1010 cfu/ μg supercoiled DNA and are ideal for high-efficiency cloning and plasmid propagation.

As a derivative of TOP10, TOP10F´ cells carry mutations in the methylation-dependent restriction system (mcrA, mcrBC, and mrr) allowing one to clone both prokaryotic and eukaryotic genomic DNA, as well as efficient plasmid rescue from eukaryotic genomes. Similar to other DH strains, the TOP10 strain also has recA1 and endA1 mutations to increase insert stability and improve the quality of plasmid DNA prepared from minipreps.

TOP10F´ Electrocomp Kit E. coli cells offer:
• Transformation efficiencies of >1 x 1010 cfu/μg
• Stable F´ episome that allows isolation of ssDNA and carries lacIq repressor for inducible expression from trc, tac, and lac promoters 
hsdR for efficient transformation of unmethylated DNA from PCR amplifications
mcrA for efficient transformation of methylated DNA from genomic preparations
lacZΔM15 for blue/white color screening of recombinant clones on plates containing either X-Gal or Bluo-Gal
endA1 for cleaner DNA preparations and better results in downstream applications due to elimination of nonspecific digestion by Endonuclease I
recA1 for reduced occurrence of nonspecific recombination in cloned DNA

Note: TOP10F´ cells require IPTG induction for blue-white color screening. A high-voltage electroporation apparatus is also required.

Genotype
F´ [lacIq, Tn10(TetR)] mcrA Δ(mrr-hsdRMS-mcrBC) φ80lacZΔM15 ΔlacX74 recA1 araD139 Δ(ara-leu)7697 galU galK rpsL(StrR) endA1 nupG

Find the strains and formats that fit your needs
We offer other DH strains in chemically competent and electrocompetent cell.
Electroporation cuvettes with 0.1 cm, 0.2 cm, and 0.4 cm gaps are available.
The TOP10 strain is available in several MultiShot formats for high throughput applications.
We offer other strains for ssDNA production, such as DH12S.
Explore bacterial growth media formats.

For Research Use Only. Not for use in diagnostic procedures.

Specifications

Product Type
Electrocompetent Cells
Improves Plasmid Quality
Yes (endA1)
Cloning Methylated DNA
Yes (mcrA)
Transformation Efficiency Level
High Efficiency (>1010 cfu/μg)
Cloning Unstable DNA
Not suitable for cloning unstable DNA
Blue/White Screening
Yes (lacZΔM15)
Plasmid
May be used for plasmids >20 kb
Preparing Unmethylated DNA
No
Reduces Recombination
Yes (recA1)
Shipping Condition
Dry Ice
Species
E. coli (K12)
Format
Tube
Product Line
Electrocomp™
Quantity
10 x 100 μL

Contents & Storage

• TOP10F' Electrocomp E. coli (10 x 100 μL)
Store at -80°C.

• pUC19 vector (50 μL at 10 pg/μL)
Store pUC19 DNA at -20°C.

• S.O.C. Medium (15 mL)
Store at room temperature.

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