Invitrogen™

TOP10F´ Electrocomp™ Kit

Catalog number:  C66511

Related applications:

Transformation

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Description

The TOP10F' genotype is identical to TOP10 with the addition of an F' episome. The F' episome carries the tetracycline resistance gene and allows isolation of single-stranded DNA from vectors that have an f1 origin of replication. In addition, the F' carries the lacIq repressor for inducible expression from trc, tac, and lac promoters using IPTG. TOP10F' require IPTG induction for blue/white color screening.

The TOP10F' genotype offers the following features:

hsdR for efficient transformation of unmethylated DNA from PCR amplifications
mcrA for efficient transformation of methylated DNA from genomic preparations
lacZΔM15 for blue/white color screening of recombinant clones
endA1 for cleaner preparations of DNA and better results in downstream applications due to the elimination of non-specific digestion by Endonuclease I
recA1 for reduced occurrence of non-specific recombination in cloned DNA
lacIq repressor for inducible expression from trc, tac, and lac promoters

Note: This kit contains enough electrocompetent cells for 25 x 40 µL rxns or 50 x 20 µL rxns. Please see the product manual for reaction scale recommendations.

Genotype:
F'{lacIqTn10(TetR)} mcrA Δ(mrr-hsdRMS-mcrBC) φ80lacZΔM15 ΔlacX74 recA1 araD139 Δ(ara-leu)7697 galU galK rpsL endA1 nupG
For Research Use Only. Not for use in diagnostic procedures.

Specifications

Bacterial or Yeast Strain: TOP10F´
Blue/White Screening: Yes
Cloning Methylated DNA: Yes
Cloning Unstable DNA: Not Suitable for cloning unstable DNA
Competent Cell Type: Electrocompetent
F'episome (to make ssDNA): Contains F' Episome
Format: Tube(s)
High-throughput Compatibility: Not High-throughput Compatible (Manual)
Improves Plasmid Quality: Yes
Plasmid: May be used for plasmids > 20 kb
Preparing Unmethylated DNA: Not Suitable for preparing unmethylated DNA
Product Line: Electrocomp™
Propagating ccdB Vectors: Not for ccdB vector propagation
Quantity: 10 x 100 μL
Reduces Recombination: Yes
Species: E. coli
T1 Phage - Resistant (tonA): No
Transformation Efficiency: > 1x10^10
Transformation Efficiency Level: High Efficiency (> 10^9 cfu⁄µg)

Contents & storage

This kit contains enough electrocompetent cells for 20 x 40 µL rxns or 40 x 20 µL rxns. Please see the product manual for reaction scale recommendations.