TOP10F´ Electrocomp™ Kit

Catalog number: C66524

Invitrogen™  Related applications: Transformation

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Description

The TOP10F' genotype is identical to TOP10 with the addition of an F' episome. The F' episome carries the tetracycline resistance gene and allows isolation of single-stranded DNA from vectors that have an f1 origin of replication. In addition, the F' carries the lacIq repressor for inducible expression from trc, tac, and lac promoters using IPTG. TOP10F' require IPTG induction for blue/white color screening.

The TOP10F' genotype offers the following features:

hsdR for efficient transformation of unmethylated DNA from PCR amplifications
mcrA for efficient transformation of methylated DNA from genomic preparations
lacZΔM15 for blue/white color screening of recombinant clones
endA1 for cleaner preparations of DNA and better results in downstream applications due to the elimination of non-specific digestion by Endonuclease I
recA1 for reduced occurrence of non-specific recombination in cloned DNA
lacIq repressor for inducible expression from trc, tac, and lac promoters

Note: This kit contains enough electrocompetent cells for 75 x 40 µL rxns or 150 x 20 µL rxns. Please see the product manual for reaction scale recommendations.

Genotype:
F'{lacIqTn10(TetR)} mcrA Δ(mrr-hsdRMS-mcrBC) φ80lacZΔM15 ΔlacX74 recA1 araD139 Δ(ara-leu)7697 galU galK rpsL endA1 nupG
For Research Use Only. Not for use in diagnostic procedures.

Specifications

Competent Cell Type: Electrocompetent
Species: E. coli
Format: Tube(s)
Transformation Efficiency: > 1x10^9
Bacterial or Yeast Strain: TOP10F´
Plasmid Type: May be used for plasmids > 20 kb
Product Size: 30 x 100 µL
Blue-White Screening: Yes
Cloning Unstable DNA: Not Suitable for cloning unstable DNA
Reduces Recombination: Yes
Cloning Methylated DNA: Yes
Improves Plasmid Quality: Yes
Propagating ccdB Vectors: Not for ccdB vector propagation
F' Episome (to make ssDNA): Contains F' Episome
Preparing Unmethylated DNA: Not Suitable for preparing unmethylated DNA
T1 Phage - Resistant (tonA): No
High Throughput Compatibility: Not High Throughput-Compatible (Manual)

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Manuals & protocols

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