Colorante CellTracker™ cm-DII
Colorante CellTracker™ cm-DII
Citas y referencias (48)
Invitrogen™

Colorante CellTracker™ cm-DII

CellTracker™ CM-DiI es un colorante fluorescente muy adecuado para supervisar el movimiento de las células o su ubicación. Este coloranteMás información
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Número de catálogoCantidad
C700020 x 50 μg
C70011mg
Número de catálogo C7000
Precio (MXN)
-
Cantidad:
20 x 50 μg
CellTracker™ CM-DiI es un colorante fluorescente muy adecuado para supervisar el movimiento de las células o su ubicación. Este colorante se conserva bien, lo que permite el seguimiento multigeneracional de movimientos celulares. Los espectros de excitación/emisión rojo oscuro son ideales para el multiplexing con colorantes fluorescentes de color verde y rojo y proteínas.

¿Necesita otro espectro de emisión o un seguimiento más prolongado? Consulte nuestros otros productos de seguimiento de células de mamífero.

• Fácil de usar: retire el medio, añada el colorante, incube 30 minutos y obtenga la imagen de las células
• Retención de señal fluorescente > 72 horas (normalmente de tres a seis generaciones)
• Espectros de excitación/emisión (553/570 nm) rojos ideales para multiplexing
• Citotoxicidad baja: no afecta a la viabilidad ni a la proliferación

El colorante fluorescente CellTracker™ CM-DiI se ha diseñado para atravesar libremente las membranas celulares hasta las células donde se transforma en productos de reacción impenetrables en células. El colorante CellTracker™ CM-DiI se mantiene en las células vivas durante varias generaciones. El tinte se transfiere a las células hijas, pero no a las células adyacentes de una población. El colorante CellTracker™ CM-DiI se ha diseñado para mostrar fluorescencia durante al menos 72 horas. Presenta unas propiedades de seguimiento idóneas: es estable, no tóxico en concentraciones de trabajo, se conserva bien en las células y muestra un brillo fluorescente con pH fisiológico. Además, los espectros de excitación y emisión del colorante CellTracker™ CM-DiI se separan fácilmente de los espectros de GFP (proteína verde fluorescente), lo que permite el multiplexing.

Para uso exclusivo en investigación. No apto para uso en procedimientos diagnósticos.

Especificaciones
ColorAmarillo
Método de detecciónFluorescente
Intervalo de longitud de onda de excitación553⁄570
Para utilizar con (equipo)Microscopio de fluorescencia
Línea de productosCellTracker
Cantidad20 x 50 μg
Condiciones de envíoTemperatura ambiente
Tipo de etiquetaProteína fluorescente
Tipo de productoTinte
SubCellular LocalizationMembranas celulares y lípidos, Lipids
Unit SizeEach
Contenido y almacenamiento
Almacenar en el congelador de -5 °C a -30 °C y proteger de la luz.

Preguntas frecuentes

I want to do a cell migration study for around 4 hours and need to fluorescently label the cells with a dye. What do you recommend?

Calcein, AM and FDA (fluorescein diaceate) are examples of some dyes used for this application. Since these dyes are not incorporated or covalently attached to any cellular components, they may have a short retention time as some cell types may actively efflux the dye out of the cells. The CellTracker and CellTrace dyes include either a mild thiol-reactive chloromethyl group or amine-reactive succinnimidyl ester group to allow for covalent binding to cellular components, providing for better retention. As with any reagent, one should empirically determine retention times for the cell type used.

Find additional tips, troubleshooting help, and resources within our Cell Analysis Support Center.

I want to perform a cell fusion assay, where one cell line is labeled with one color and the other cell line with another color, and combine with a nucleic acid stain. What do you recommend?

A typical method is to label one cell line with orange fluorescent DiI C18 and the other cell line with green fluorescent DiO C18. These orange and green lipophilic cyanine dyes will stain the membranes of cells. Cells that fuse will then have both dyes, yielding a yellow color (when images are overlaid or cells are imaged in a dual-bandpass filter). These live cells can then be labeled with Hoechst 33342 (a cell-permeant blue DNA stain comparable in wavelength to DAPI), but only as an endpoint just before imaging (since DNA stains can interrupt DNA function).

Find additional tips, troubleshooting help, and resources within our Cell Analysis Support Center.

I need to look at live cell morphology deformation over the course of a few hours. What sort of membrane dye would be useful for this?

Lipophilic cyanine dyes, such as DiI (Cat. No. D282), DiO (Cat. No. D275), DiD (Cat. No. D7757) or DiR (Cat. No. D12731), are commonly used. The longer the alkyl chain on the dye, the better the retention in lipophilic environments.

Find additional tips, troubleshooting help, and resources within our Cell Analysis Support Center.

I labeled my cells with Calcein, AM, but when I imaged the next day, there was no fluorescence from Calcein. Why?

Calcein, AM is a good choice for cell tracking and as a general cytoplasmic stain. However, it doesn't bind to anything and may be actively pumped out of the cells within a couple hours, which is likely what happened. The retention of Calcein within live cells is dependent upon the inherent properties of the cell type and culture conditions.

For long-term imaging, you may wish to consider a reactive cytoplasmic stains such as CFDA, SE or the CellTracker and CellTrace dyes.

Find additional tips, troubleshooting help, and resources within our Cell Analysis Support Center.

How do I prepare a stock solution of CellTracker CM-DiI Dye (Cat. No. C7000, C7001)?

A stock solution of CellTracker CM-DiI Dye (Cat. No. C7000, C7001) may be prepared in dimethyl formamide (DMF), dimethylsulfoxide (DMSO), or ethanol at a concentration of 1-2 mg/mL.

Find additional tips, troubleshooting help, and resources within our Cell Analysis Support Center.

View all Colorante CellTracker™ cm-DII FAQs

Citations & References (48)

Citations & References
Abstract
The use of the lipophilic fluorochrome CM-DiI for tracking the migration of lymphocytes.
Authors:Andrade W, Seabrook TJ, Johnston MG, Hay JB
Journal:J Immunol Methods
PubMed ID:8765171
'In this study we examined the new cell dye CM-DiI for tracking the migration of lymphocytes from blood to lymph. This lipophilic marker intercalates in the plasma membrane like the PKH dyes and older DiI derivatives. The stability and intensity of staining achieved with these dyes is better than most ... More
Characterization of antisera specific to NK1, NK2, and NK3 neurokinin receptors and their utilization to localize receptors in the rat gastrointestinal tract.
Authors:Grady EF, Baluk P, Böhm S, Gamp PD, Wong H, Payan DG, Ansel J, Portbury AL, Furness JB, McDonald DM, Bunnett NW
Journal:J Neurosci
PubMed ID:8824334
'Understanding the physiological role of tachykinins requires precise cellular and subcellular localization of their receptors. We raised antisera by immunizing rabbits with peptides corresponding to portions of the intracellular tails of the rat neurokinin 1, 2, and 3 receptors (NK1-R, NK2-R, NK3-R). Receptors were localized by immunofluorescence and confocal microscopy. ... More
Interneuron migration from basal forebrain to neocortex: dependence on Dlx genes.
Authors:Anderson SA, Eisenstat DD, Shi L, Rubenstein JL
Journal:Science
PubMed ID:9334308
'Although previous analyses indicate that neocortical neurons originate from the cortical proliferative zone, evidence suggests that a subpopulation of neocortical interneurons originates within the subcortical telencephalon. For example, gamma-aminobutyric acid (GABA)-expressing cells migrate in vitro from the subcortical telencephalon into the neocortex. The number of GABA-expressing cells in neocortical slices ... More
Analysis of vertical fluorescence resonance energy transfer from the surface of a small-diameter sphere.
Authors:Jones GM, Wofsy C, Aurell C, Sklar LA
Journal:Biophys J
PubMed ID:9876165
'Fluorescence resonance energy transfer (FRET) measurements have been used to analyze fluorophore separations in a number of varying geometries, including small particles and extended surfaces. This study focuses on the geometry created by a donor extended above the surface of a small sphere (radius < R0), where the acceptors are ... More
Human mesenchymal stem cells exert potent antitumorigenic effects in a model of Kaposi's sarcoma.
Authors:Khakoo AY, Pati S, Anderson SA, Reid W, Elshal MF, Rovira II, Nguyen AT, Malide D, Combs CA, Hall G, Zhang J, Raffeld M, Rogers TB, Stetler-Stevenson W, Frank JA, Reitz M, Finkel T,
Journal:J Exp Med
PubMed ID:16636132
'Emerging evidence suggests that both human stem cells and mature stromal cells can play an important role in the development and growth of human malignancies. In contrast to these tumor-promoting properties, we observed that in an in vivo model of Kaposi''s sarcoma (KS), intravenously (i.v.) injected human mesenchymal stem cells ... More
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