Invitrogen™

One Shot™ Mach1™ T1 Phage-Resistant Chemically Competent E. coli

Catalog number: C862003
Invitrogen™

One Shot™ Mach1™ T1 Phage-Resistant Chemically Competent E. coli

Catalog number: C862003
Catalog Number
C862003
also known as C8620-03
Unit Size
21 x 50 µL/tube
Price (USD)
Price: 476.00
Your Price:
Availability
***
Quantity
Catalog NumberUnit SizePrice (USD)AvailabilityQuantity
C862003
also known as C8620-03
21 x 50 µL/tube
Price: 476.00
Your Price:
***
Product Overview
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Additional Information
Recommendations
Recommendations
One Shot® Mach1™ T1 Phage-Resistant Chemically Competent E. coli are the fastest growing chemically competent cells currently available (see figure). Doubling time is approximately 50 minutes, compared to >74 minutes for other cloning strains. Mach1™ colonies are clearly visible within eight hours of plating the transformation mix, enabling you to plate and pick colonies the same day. Perform mini-preps after only 4 hours of growth from an overnight colony—saving an entire day in your experiments. The fast-growing Mach1™ T1R strain:

• Minimizes nonspecific recombination in cloned DNA (recA)
• Offers resistance to T1 and T5 phage (tonA)
• Yields cleaner preparations of DNA and better results in downstream applications due to the elimination of nonspecific digestion by Endonuclease I (endA1)
• Provides efficient transformation of unmethylated DNA from PCR amplifications (hsdR)
• Allows blue/white screening of recombinant clones (lacZΔM15)

Two formats to choose from
• The convenient One Shot® format provides single-use, 50-µL aliquots that eliminate efficiency-zapping, freeze-thaw cycles and money wasted on unused cells
• The MultiShot™ StripWell format allows rapid, high-throughput transformation in a 96-well stripwell plate, which allows you to perform as few or as many transformations as you need without wasting reagents
For Research Use Only. Not for use in diagnostic procedures.

Specifications

Product Type
Competent Cell
Contains F' Episome
Lacks F' Episome
Efficiency
High Efficiency (>10^9 cfu/µg)
Cell Type
Chemically Competent
Improves Plasmid Quality
Yes
Cloning Methylated DNA
No
Transformation Efficiency Level
High Efficiency (> 10^9 cfu⁄µg)
Cloning Unstable DNA
Not Suitable for cloning unstable DNA
Bacterial or Yeast Strain
Mach1™
Blue/White Screening
Yes
High-throughput Compatibility
Not High-throughput Compatible (Manual)
Preparing Unmethylated DNA
Not Suitable for preparing unmethylated DNA
Propagating ccdB Vectors
Not for ccdB vector propagation
Reduces Recombination
Yes
Shipping Condition
Dry Ice
T1 Phage - Resistant (tonA)
Yes
Species
E. coli
Format
One Shot
Product Line
One Shot™
Quantity
20 x 50 μL

Contents & Storage

Contains:
• One Shot™ Mach1™-T1R Chemically Competent E. coli: 21 vials, 50 µl each
• pUC19 DNA (10 pg/µl): 1 vial, 50 µl
• S.O.C. medium: 1 bottle, 6 ml

Store Competent Cells at -80°C. Store pUC19 DNA at -20°C. Store SOC Medium at 4°C or at room temperature.

Figures