MultiShot™ FlexPlate Mach1™ T1R Competent Cells

Description

MultiShot FlexPlate Mach1 T1^R chemically competent E. coli cells are cloning competent cells that are pre-aliquoted in a 96-well PCR plate to increase transformation productivity. The FlexPlate format can be divided down to any combination of 8-well segments for flexibility in transformation throughput. MultiShot FlexPlate Mach1 T1^R competent E. coli cells are exceptionally fast-growing chemically competent cells, accelerating the time to clone evaluation.

All of our T1^R strains have the tonA (fhuA) deletion in their genotype that confers resistance to T1 and T5 phage. T1 bacteriophage spread rapidly and lyse E. coli hosts, which are commonly used for cloning and library construction. A resistant genotype offers added security to protect valuable clones and libraries. This is especially important for genome and sequencing centers where T1 phage infection would be catastrophic.

Speedy Mach1 T1^R cloning capabilities
MultiShot FlexPlate Mach1 T1^R competent E. coli cells are fast growing. Mach1 colonies are clearly visible within eight hours of plating the transformation mix, enabling you to plate and pick colonies the same day. Perform mini-preps after only four hours of growth from an overnight colony—saving an entire day in your experiments. The Mach1 T1^R strain is useful for many cloning applications and provides transformation efficiencies of >1 x 10⁸ cfu/µg with control plasmid DNA.

Key features of the MultiShot FlexPlate Mach1 T1^R Competent Cells include:
• endA1 for cleaner preparations of DNA and better results in downstream applications due to the elimination of non-specific digestion by endonuclease I
• recA1398 for reduced occurrence of non-specific recombination in cloned DNA
• hsdR for efficient transformation of unmethylated DNA from PCR amplifications
• tonA (fhuA) for T1 and T5 phage resistance
• lacZΔM15 for blue/white color screening of recombinant clones

MultiShot FlexWell format—increase transformation productivity
The FlexPlate is a 96-well PCR plate that can be used in automated liquid handling systems or divided into any combination of 8-well segments for flexibility in transformation throughput. Addition of DNA to the MultiShot FlexPlate Competent Cells can be done quickly with a multichannel pipet or a liquid handling system. After addition of DNA, MultiShot FlexPlate Competent Cells can efficiently be transformed by heat-shock using a water bath, heat block, or thermal cycler.

Key features of the MultiShot FlexPlate format include:
• Increase throughput—compatible with automated liquid handling platforms
• Flexible—transform in increments of 8 wells to entire 96-well plate
• Convenient—competent cells pre-aliquoted into 96-well plate, ready for transformation

Genotype: F- φ80(lacZ)ΔM15 ΔlacX74 hsdR(rK-mK+) ΔrecA1398 endA1 tonA

Find the strain and format that you need
We offer many other E. coli strains and formats of chemically competent cells and electrocompetent cells to help meet your specific transformation needs. If you require other high-throughput transformation options, choose from our collection of MultiShot formatted comp cells or email us for custom formatting options.

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Specifications

Bacterial or Yeast Strain:	Mach1™ T1
Blue/White Screening:	Yes
Cloning Methylated DNA:	No
Competent Cell Type:	Chemically Competent
F'episome (to make ssDNA):	Lacks F' Episome
High-throughput Compatibility:	High-throughput Compatible
Product Line:	Mach1™, MultiShot™
Propagating ccdB Vectors:	Not for ccdB vector propagation
Shipping Condition:	Dry Ice
Format:	96-well plate
Species:	E. coli

Contents & storage