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OriGene
VIM Monoclonal Antibody (OTI3B11), TrueMAB™
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FIGURE: 1 / 3
VIM Antibody (CF801221) in WB
HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY VIM (RC201546, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 µg per lane) were separated by SDS-PAGE and immunoblotted with anti-VIM. Positive lysates LY401165 (100 µg) and LC401165 (20 µg) can be purchased separately from OriGene.
Product Details
CF801221
Product Specifications
Species Reactivity
Human
Host/Isotype
Mouse
/ IgG1
Class
Monoclonal
Type
Antibody
Clone
OTI3B11
Immunogen
Human recombinant protein fragment corresponding to amino acids 210-466 of human VIM produced in E.coli.
Conjugate
Form
Lyophilized
Concentration
1 mg/mL
Purification
Affinity chromatography
Storage buffer
PBS, pH 7.3, with 8% trehalose
Contains
no preservative
Storage conditions
-20°C, Avoid Freeze/Thaw Cycles
Shipping conditions
Ambient (domestic); Wet ice (international)
Product Specific Information
For reconstitution, we recommend adding 100 µL distilled water to a final antibody concentration of about 1 mg/mL. To use this carrier-free antibody for conjugation experiments, we strongly recommend performing another round of desalting. (Zeba Spin Desalting Columns, 7KMWCO, 0.5 mL, Product # 89882)
Target Information
Vimentin is a developmentally regulated intermediate filament protein (IFP) found in cells of mesenchymal origin. It is believed to be involved with the intracellular transport of proteins between the nucleus and plasma membrane. Unlike other IFP proteins, vimentin is expressed, along with desmin, during the early stages of cellular development. During the development process, vimentin is exchanged for new, tissue-specific IFPs. Vimentin has been implicated to be involved in the rate of steroid synthesis via its role as a storage network for steroidogenic cholesterol containing lipid droplets. Vimentin phosphorylation by a protein kinase causes the breakdown of intermediate filaments and activation of an ATP and myosin light chain dependent contractile event. This results in cytoskeletal changes that facilitate the interaction of the lipid droplets within mitochondria, and subsequent transport of cholesterol to the organelles leading to an increase in steroid synthesis.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: epididymis secretory sperm binding protein; FLJ36605; Hel113; RP11-124N14.1; unnamed protein product; VIM; VIME; Vimentin
Gene Aliases: VIM
UniProt ID: (Human) P08670
Entrez Gene ID: (Human) 7431
positive regulation of gene expression
negative regulation of neuron projection development
astrocyte development
neuron projection development
positive regulation of collagen biosynthetic process
regulation of mRNA stability
intermediate filament-based process
intermediate filament organization
Bergmann glial cell differentiation
lens fiber cell development
cellular response to lipopolysaccharide
cellular response to muramyl dipeptide
cellular response to interferon-gamma
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Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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