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OriGene
SETD2 Monoclonal Antibody (OTI1B4), TrueMAB™
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Product Details
CF804128
Product Specifications
Species Reactivity
Human
Host/Isotype
Mouse
/ IgG1
Class
Monoclonal
Type
Antibody
Clone
OTI1B4
Immunogen
Human recombinant protein fragment corresponding to amino acids 1787-2144 of human SETD2 produced in E.coli.
Conjugate
Form
Lyophilized
Concentration
1 mg/mL
Purification
Affinity chromatography
Storage buffer
PBS, pH 7.3, with 8% trehalose
Contains
no preservative
Storage conditions
-20°C, Avoid Freeze/Thaw Cycles
Shipping conditions
Ambient (domestic); Wet ice (international)
Product Specific Information
For reconstitution, we recommend adding 100 µL distilled water to a final antibody concentration of about 1 mg/mL. To use this carrier-free antibody for conjugation experiments, we strongly recommend performing another round of desalting. (Zeba Spin Desalting Columns, 7KMWCO, 0.5 mL, Product # 89882)
Target Information
Histone methyltransferase that specifically trimethylates 'Lys-36' of histone H3 (H3K36me3) using dimethylated 'Lys-36' (H3K36me2) as substrate. Represents the main enzyme generating H3K36me3, a specific tag for epigenetic transcriptional activation. Plays a role in chromatin structure modulation during elongation by coordinating recruitment of the FACT complex and by interacting with hyperphosphorylated POLR2A. Acts as a key regulator of DNA mismatch repair in G1 and early S phase by generating H3K36me3, a mark required to recruit MSH6 subunit of the MutS alpha complex: early recruitment of the MutS alpha complex to chromatin to be replicated allows a quick identification of mismatch DNA to initiate the mismatch repair reaction. H3K36me3 also plays an essential role in the maintenance of a heterochromatic state, by recruiting DNA methyltransferase DNMT3A. H3K36me3 is also enhanced in intron-containing genes, suggesting that SETD2 recruitment is enhanced by splicing and that splicing is coupled to recruitment of elongating RNA polymerase. Required during angiogenesis. Recruited to the promoters of adenovirus 12 E1A gene in case of infection, possibly leading to regulate its expression.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: contains WW domain; FLJ16420; FLJ22472; FLJ23184; FLJ45883; HIF-1; HIP-1; Histone-lysine N-methyltransferase SETD2; hSET2; huntingtin interacting protein 1; Huntingtin yeast partner B; Huntingtin-interacting protein 1; Huntingtin-interacting protein B; HYPB; contains WW domain; Lysine N-methyltransferase 3A; p231HBP; Protein-lysine N-methyltransferase SETD2; SET domain-containing protein 2; unnamed protein product
Gene Aliases: HBP231; HIF-1; HIF1; HIP-1; HSPC069; HYPB; KIAA1732; KMT3A; LLS; MRD70; p231HBP; RAPAS; SET2; SETD2
UniProt ID: (Human) Q9BYW2
Entrez Gene ID: (Human) 29072
protein binding
methyltransferase activity
protein-lysine N-methyltransferase activity
transferase activity
histone methyltransferase activity
alpha-tubulin binding
metal ion binding
histone methyltransferase activity (H3-K36 specific)
histone H3 methyltransferase activity
histone H3K36 trimethyltransferase activity
autophagosome assembly
immune system process
DNA repair
mismatch repair
chromatin organization
regulation of transcription, DNA-templated
transcription elongation from RNA polymerase II promoter
cellular response to DNA damage stimulus
response to metal ion
regulation of gene expression
proteasomal protein catabolic process
positive regulation of autophagy
regulation of double-strand break repair via homologous recombination
regulation of mRNA export from nucleus
peptidyl-lysine trimethylation
cell differentiation
methylation
regulation of cytokinesis
positive regulation of interferon-alpha production
response to type I interferon
nucleosome organization
endodermal cell differentiation
response to alkaloid
innate immune response
positive regulation of ossification
stem cell differentiation
defense response to virus
protein K48-linked ubiquitination
microtubule cytoskeleton organization involved in mitosis
regulation of protein localization to chromatin
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Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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