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OriGene
JAK3 Monoclonal Antibody (OTI2B4), TrueMAB™
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FIGURE: 1 / 1
JAK3 Antibody (CF812875) in WB
HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY JAK3 (RC217928, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 µg per lane) were separated by SDS-PAGE and immunoblotted with anti-JAK3. (1. Positive lysates LY424859 (100 µg) and LC424859 (20 µg) can be purchased separately from OriGene.
Product Details
CF812875
Product Specifications
Species Reactivity
Human
Host/Isotype
Mouse
/ IgG1
Class
Monoclonal
Type
Antibody
Clone
OTI2B4
Immunogen
Human recombinant protein fragment corresponding to amino acids 811-1124 of human JAK3 produced in E.coli.
Conjugate
Form
Lyophilized
Concentration
1 mg/mL
Amount
100 µg
Purification
Affinity chromatography
Storage buffer
PBS, pH 7.3, with 8% trehalose
Contains
no preservative
Storage conditions
-20°C, Avoid Freeze/Thaw Cycles
Shipping conditions
Ambient (domestic); Wet ice (international)
Product Specific Information
For reconstitution, we recommend adding 100 µL distilled water to a final antibody concentration of about 1 mg/mL. To use this carrier-free antibody for conjugation experiments, we strongly recommend performing another round of desalting. (Zeba Spin Desalting Columns, 7KMWCO, 0.5 mL, Product # 89882)
Target Information
Janus kinase 3 (JAK3) phosphorylates members of the STAT (signal transduction and activators of transcription) family. JAK3 is predominantly expressed in immune cells and transduces a signal in response to activation of interleukin receptors. In response to a varitety of cytokine or related factors (e.g., interferon, interleukins), JAKs are activated via phosphorylation at 2 adjacent tyrosine residues. The activation of JAKs can lead to the phosphorylation of STAT (signal transducers and activatiors of transcription) proteins, which dimerize and translocate to the nucleus. Once translocated to the nucleus, the STAT proteins can modify transcription of numerous genes, including interferon-stimulated genes. JAK2 is required for the IFN gamma-receptor complex initiation and JAK1 functions as an amplifier. However, active JAK1 may be required for complex responses. Some studies have suggested that the role of JAK2 might be performed by Tyk2 and JAK3, if they were positioned correctly within the IFN gamma-receptor complex.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: Breast-JAK3; JAK-3; JAK3B; Janus kinase 3; Janus kinase 3 (a protein tyrosine kinase, leukocyte); L JAK; L-JAK; Leukocyte janus kinase; Tyrosine-protein kinase JAK3; unnamed protein product
Gene Aliases: JAK-3; JAK3; JAK3_HUMAN; JAKL; L-JAK; LJAK
UniProt ID: (Human) P52333
Entrez Gene ID: (Human) 3718
adaptive immune response
immune system process
negative regulation of dendritic cell cytokine production
enzyme linked receptor protein signaling pathway
JAK-STAT cascade
tyrosine phosphorylation of STAT protein
negative regulation of glycoprotein biosynthetic process
cytokine-mediated signaling pathway
cell differentiation
B cell differentiation
negative regulation of interleukin-10 production
negative regulation of interleukin-12 production
intracellular signal transduction
interleukin-15-mediated signaling pathway
interleukin-4-mediated signaling pathway
interleukin-2-mediated signaling pathway
interleukin-7-mediated signaling pathway
interleukin-9-mediated signaling pathway
regulation of apoptotic process
T cell homeostasis
innate immune response
negative regulation of T-helper 1 cell differentiation
regulation of JAK-STAT cascade
regulation of alpha-beta T cell activation
negative regulation of T cell activation
JAK-STAT cascade involved in growth hormone signaling pathway
regulation of T cell apoptotic process
negative regulation of thymocyte apoptotic process
response to interleukin-2
response to interleukin-4
response to interleukin-15
response to interleukin-9
regulation of leukocyte cell-cell adhesion
negative regulation of T-helper 17 cell lineage commitment
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Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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