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Invitrogen™

Cancer Cell Isolation Kit

Catalog number:  CI0010

 Related applications:

Cell Isolation & Expansion

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Description

The Cancer Cell Isolation Kit provides a simple, cost-effective method for isolating cancer cells from biopsy samples. Cancer cells purified using this technique can be used immediately for a wide range of applications, including biomarker identification, microarray hybridization, and genetic analysis. Moreover, the whole cell isolation procedure is so gentle that cells can be used to establish primary cell lines.

• Purify cancer cells from fresh biopsy samples
• Establish primary cell lines or use for biochemical assays
• Ideal for proteomics applications

Purify any population of cancer cells
The ability to obtain uncontaminated cancer cell samples is one of the major bottlenecks in the study of tumor development and cancer biology. Tumor biopsies from cancer patients and animal tumor models often contain a heterogeneous population of cells that include normal tissue, blood, and cancer cells. This mixed population makes diagnosis and valid experimental conclusions difficult to obtain and interpret.

Typically, cancer cells are isolated from biopsy samples via microdissection, a time-consuming technique that requires costly equipment and surgical precision. Another conventional method is immunoprecipitation with antibodies specific to each cancer cell type. Unlike these techniques, the USB Cancer Cell Isolation Kit requires no specialized skill set and no expensive equipment, and can be used to isolate many cancer cell types.

Simple, cost-effective technique
The cancer cell isolation method only takes a couple of hours, and involves just a few rounds of incubation, washing, and centrifugation. Using the same amount of starting biopsy material required for conventional methods, this technique can be used to isolate a much greater quantity of cancer cells.

Cancer cells purified using the Cancer Cell Isolation Kit can be used directly for proteomics applications such as identification of biomarkers or for RNAbased applications such as microarray hybridization and genetic analysis. Cells can also be used to purify proteins to test antibody-based cancer therapies. Alternatively, cells can be used to establish primary cell lines.

To prepare nuclear extracts from isolated cancer cells, please see the Nuclear Extraction Kit, PN AY2002.
For Research Use Only. Not for use in diagnostic procedures.

Specifications

Cell Type: Cancer
Product Size: 10 assays

Contents & storage

Tumor cell suspension solution
Tumor cell digestion solution
Tumor cell isolation solution
100-µm filter

Documents

Manuals & protocols