Thermo Scientific™

Reinforced Clostridial Medium (RCM) (Dehydrated)

Cultivate and enumerate clostridia and other anaerobes occurring in food and pathological samples with Thermo Scientific™ Oxoid™ Reinforced Clostridial Medium. A semi-solid, highly nutritious medium can be used as an enrichment medium to optimize the growth of clostridia before sub-culturing to solid agar.
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Use Reinforced Clostridial Medium (RCM) for the isolation and cultivation of anaerobic organisms occurring in a variety of habitats, including food and pathological specimens.

  • Superior fertility: the medium produced the highest viable count when compared to other media tested by Hirsch & Grinstead 1
  • Differential: can be made differential for sulphite-reducing clostridia by the addition of sodium sulphite and ferric citrate2

Reinforced Clostridial Medium (RCM) was designed by Hirsch & Grinstead1 for the cultivation and enumeration of clostridia. They showed that the medium was more fertile and enabled growth to be initiated from small inocula more readily than five other media tested. In a further comparison, the highest viable count obtainable was the criterion used, and again, RCM proved superior. Compared with the spleen infusion medium of Mundt & Jones3, RCM gave somewhat higher counts (Gibbs & Hirsch4).

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Remel™ and Oxoid™ products are now part of the Thermo Scientific brand.

For In Vitro Diagnostic Use.  
1. Hirsch A. and Grinstead E. (1954) J. Dairy Re+AH3:AH11s. 21. 101-110.

2. Gibbs B.M. and Freame B. (1965) J. Appl. Bact. 28. 95-111.

3. Mundt J. O. and Jones V.W. (1952) Bact. Proc. p. 106.

4. Gibbs B.M. and Hirsch A. (1956) J. Appl. Bact. 19. 129-141.