Use Reinforced Clostridial Medium (RCM) for the isolation and cultivation of anaerobic organisms occurring in a variety of habitats, including food and pathological specimens.
Reinforced Clostridial Medium (RCM) was designed by Hirsch & Grinstead1 for the cultivation and enumeration of clostridia. They showed that the medium was more fertile and enabled growth to be initiated from small inocula more readily than five other media tested. In a further comparison, the highest viable count obtainable was the criterion used, and again, RCM proved superior. Compared with the spleen infusion medium of Mundt & Jones3, RCM gave somewhat higher counts (Gibbs & Hirsch4).
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