Differentiate Enterobacteriaceae according to their ability to ferment lactose, sucrose, and glucose, and to produce hydrogen sulphide using Thermo Scientific™ Oxoid™ Triple Sugar Iron Agar (Dehydrated). The Triple Sugar Iron Agar is based on Kligler Iron Agar, additionally, it contains sucrose for the detection of sucrose fermenting species.
The composite medium contains Peptone, 'Lab-Lemco' powder and yeast extracts to provide nitrogenous compounds and trace elements while sodium chloride maintains osmotic equilibrium. Lactose, sucrose and glucose are the fermentable carbohydrates. Sodium thiosulphate and ferric ions make the H2S indicator system while Phenol red is the pH indicator.
Bacteria that ferment any of the three sugars in the medium will produce by-products. These by-products are usually acids, which will change the color of the phenol red to yellow. Position of the color change distinguishes the acid production associated with glucose fermentation from the acidic by-products of lactose or sucrose fermentation. Many bacteria that can ferment sugars in the anaerobic butt of the tube are enterobacteria.
Typical reactions of organisms in Triple Sugar Iron Agar:
|Escherichia coli ATCC® 8739 *||A||A||+||-|
|Proteus hauseri ATCC® 13315*||A||A||+||+|
|Pseudomonas aeruginosa ATCC® 9027 *||ALK||ALK||-||-|
|Salmonella enteritidis ATCC® 13076 *||ALK||ALK||+||+|
Triple Sugar Iron Agar is recommended for the presumptive identification of colonies or sub-cultures from plating media such as Salmonella Shigella Agar (Modified) (CM0533), Bismuth Sulphite Agar (CM0201), Brilliant Green Agar (CM0263), MacConkey Agar No.3 (CM0115), or Desoxycholate Citrate Agar (Hynes) (CM0227).
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