Thermo Scientific™

Mueller Hinton Agar (Dehydrated)

 Related applications:
Industrial Microbiology

Perform effortless Antimicrobial Susceptibility Testing (AST) using Thermo Scientific™ Oxoid™ Mueller Hinton Agar (Dehydrated), a medium designed and manufactured according to the European standard, EUCAST1 and the American standard, CLSI2, 3, 4.

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Description

The major use of Mueller-Hinton Agar is for Antimicrobial Susceptibility Testing (AST). It has become the standard medium for the Kirby-Bauer method5, 6 and can be used in internationally recognized standard procedures.

  • Specified by the Clinical and Laboratory Standards Institute (CLSI) formerly the National Committee for Clinical Laboratory Standards (NCCLS) 2,3,4 and The European Committee on Antimicrobial Susceptibility Testing (EUCAST)1
  • Standard medium for the Kirby-Bauer method5, 6

The inclusion of starch in the medium ensures that toxic factors produced during microbial growth will be absorbed, and its presence is often essential to establish growth from very small inocula.

Antibiotic susceptibility tests are performed in accordance with and meet the acceptance limits of the current ISO/TS 16782 using the following microorganisms:

Staphylococcus aureus ATCC®25923 WDCM00034
Staphylococcus aureus ATCC®29213 WDCM00131
Staphylococcus aureus ATCC®43300 WDCM00211
Staphylococcus aureus NCTC 12493 WDCM00212
Escherichia coli ATCC®25922 WDCM00013
Escherichia coli ATCC®35218
Pseudomonas aeruginosa ATCC®27853 WDCM00025
Enterococcus faecalis ATCC®33186 WDCM00210
Enterococcus faecalis ATCC®29212 WDCM00087
Streptococcus pneumoniae ATCC®49619
Haemophilus influenzae ATCC®49247
Haemophilus influenzae ATCC®49766

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Remel™ and Oxoid™ products are now part of the Thermo Scientific brand

For Laboratory Use Only

1. European Committee on Antimicrobial Susceptibility Testing. EUCAST disc diffusion Test Methodology www.eucast.org

2. CLSI Performance Standards for Antimicrobial Disk Susceptibility Testing; CLSI Supplement M100S Wayne PA: Clinical and Laboratory Standards Institute

3. CLSI Performance Standards for Antimicrobial Susceptibility Tests; Approved Standard. CLSI Document M02. Wayne PA: Clinical and Laboratory Standards Institute

4. Pollock H. M. et al. (1986) J. Clin. Microbiol. 24. 1-6.

5. Bauer A. W., Kirby W. M., Sherris J. C. and Turck M. (1966) Amer. J. Clin. Path. 45. 493-496.

6. Ryan K. J., Schoenknecht F. D. and Kirby W. M. (1970) Hosp. Pract. 5. 91-100.



Documents

Manuals & protocols