Thermo Scientific™

Schaedler Anaerobe Agar (Dehydrated)

Grow and isolate aerobic and anaerobic organisms of the intestinal tract, which may require selective conditions to inhibit the growth of background organisms like Escherichia coli, with Thermo Scientific™ Oxoid™ Schaedler Anaerobe Agar. The presence of cysteine in the medium provides selectivity against Escherichia coli as cysteine has shown to have inhibitory effects on several enzymatic reactions of Escherichia coli in vitro.1
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Schandler Anaerobe Agar is free from thioglycollate and is used to create selective conditions under which delicate and more nutritionally exacting microorganisms of the intestinal tract develop, despite the presence of antagonistic organisms. Normally such fastidious microorganisms would be swamped by the growth of enterococci, coliform, bacilli, and other Gram-negative bacilli.

 

  • Selective against non-target Gram-negative bacteria, especially Escherichia coli

 

Schaedler, Dubos, and Costello3 formulated this medium for the isolation of aerobic and anaerobic microorganisms from the gastro-intestinal tract of mice. Mata, Carrillo, and Villatoro4 modified the formula in their studies on anaerobic human fecal microflora.

 

Although thioglycollate is widely used in anaerobic media, to lower the redox potential in order to favor growth of anaerobic organisms, some workers have reported it to be inhibitory to some anaerobes.2,3

 

Schaedler Anaerobe Agar contains cysteine hydrochloride and glucose, as reducing substances, with the advantage that cysteine inhibits the growth of Escherichia coli. Kari, Nagy, Kovacs & Hernadi4 have reported the inhibitory effect of cysteine on several enzymatic reactions of Escherichia coli in vitro.

 

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Remel™ and Oxoid™ products are now part of the Thermo Scientific brand.

For In Vitro Diagnostic Use.  
1. Kari C., Nagy Z., Kovacs P. and Hernadi F. (1971) J. Gen. Micro. 68. 349-356.
2. de Waart J. (1973) Personal Communication.
3. Schaedler R. W., Dubos R. and Castello R. (1965) J. Exp. Med. 122. 59-66.
4. Mata L. J., Carrillo C. and Villatoro E. (1969) Appl. Microbiol. 17. 596-599.