Use Alkaline Saline Peptone Water (ISO) for selective recovery and enrichment of Vibrio species.
Alkaline Peptone Water was first formulated by Shread, Donovan and Lee1 to be used as a non-selective enrichment broth for the cultivation of Aeromonas spp. Cruickshank reported that when the pH is raised, the medium can be used to effectively cultivate Vibrio species.2 The 2% (w/v) sodium chloride incorporated in this medium promotes the growth of Vibrio cholerae, while the alkalinity of this medium inhibits most of the unwanted background flora.
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