Thermo Scientific™

Oxoid™ ONE Broth-Listeria

Catalog number: CM1066R
Thermo Scientific™

Oxoid™ ONE Broth-Listeria

Catalog number: CM1066R

Oxoid ONE Broth-Listeria is a selective enrichment broth for Listeria species in food samples in 24 hours. Novel enrichment broth balances a mix of peptones, carbohydrates and salts for optimal resuscitation, recovery and growth of even low numbers of Listeria spp.

 
Catalog Number
CM1066R
Unit Size
Each
Quantity
2.5 kg
Price (USD)
Full specifications
FormatPowder
TypeBroth
DescriptionONE Broth-Listeria
Product TypeDehydrated Culture Media
YieldFor 57L medium
Quantity2.5 kg
Unit SizeEach
Catalog Number
Unit Size
Each
Quantity
500 g
Price (USD)
Full specifications
FormatPowder
TypeBroth
DescriptionONE Broth-Listeria
Product TypeDehydrated Culture Media
YieldFor 11.4L medium
Quantity500 g
Unit SizeEach
Showing 2 of 2
Catalog NumberSpecificationsUnit SizeQuantityPrice (USD)
CM1066RFull specifications
Each2.5 kgRequest A Quote
FormatPowder
TypeBroth
DescriptionONE Broth-Listeria
Product TypeDehydrated Culture Media
YieldFor 57L medium
Quantity2.5 kg
Unit SizeEach
CM1066BFull specifications
Each500 gRequest A Quote
FormatPowder
TypeBroth
DescriptionONE Broth-Listeria
Product TypeDehydrated Culture Media
YieldFor 11.4L medium
Quantity500 g
Unit SizeEach
Showing 2 of 2

Requires ONE Broth-Listeria Supplement, Part No. SR00234E.

ONE Broth - Listeria: Typical Formulation

  • Peptone: 26g/L; Carbohydrate mix: 6g/L; Salt mix: 10g/L with a final pH of 7.4 ±0.2 at 25°C

Appearance:

  • Dehydrated medium: straw coloured, free-flowing powder
  • Selective supplement: orange/yellow, freeze-dried pellet
  • Prepared medium: dark straw coloured, clear liquid

Formulation Directions

  • Suspend 22g of ONE Broth-Listeria Base in 500ml (44g/L) of distilled water
  • Mix well and sterilize by autoclaving at 121°C for 15 minutes
  • Cool the medium to below 50°C and aseptically add the contents of one vial of ONE Broth-Listeria Selective Supplement (Mfr. No. SR0234) reconstituted as directed

Testing Protocol

  • Add 25g of food sample to 225mL of ONE Broth-Listeria and stomach for a minimum of 30 seconds to mix the sample
  • Incubate the broth without agitation at 30°C for 24 hours
  • Gently agitate the bag then, using a microbiological loop, remove 10μL and inoculate onto a suitable agar medium e.g. Brilliance Listeria Agar (Mfr. No. CM1080, SR0222, and SR0228) and incubate appropriately (37°C for 24-48 hours)
  • Confirm presumptive colonies on the agar plates as Listeria monocytogenes or Listeria spp. by appropriate methods e.g. Gram-stain, catalase, oxidase, Oxoid Listeria Latex Test Kit (Mfr. No. DR1126)
  • Oxoid O.B.I.S. mono (Mfr. No. ID0600), Microbact Listeria 12L (Mfr. No. MB1128)
  • For Laboratory Use Only

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