Cronobacter sakazakii is a Gram-negative rod-shaped bacterium that rarely causes disease in healthy adults. It has been implicated in outbreaks of disease in premature infants (neonates). Research suggests that neonates, or those infants who have other medical conditions, are more susceptible to this infection. Most reported cases of infection are severe, including sepsis (bacteria in the blood, meningitis, or necrotizing enterocolitis (severe intestinal infection).

Use Chromogenic Cronobacter Isolation Agar for differentiation and enumeration of Cronobacter sakazakii from infant formula and other food samples.

• Selective: due to the presence of desoxycholate the medium is selective against most Gram-positive organisms.
• Easy-to-read: Cronobacter sakazakii produces blue-green colonies on this medium.

Cronobacter spp. have been isolated at low levels from powdered infant formulae, and the organisms’ high tolerance to desiccation provides a competitive advantage in the dry environments of milk powder factories, increasing the risk of product contamination¹.

Chromogenic Cronobacter Isolation (CCI) agar is an optimisation of the original Brilliance™ Enterobacter Sakazakii Agar (DFI), developed by Iversen et al. in 2004³. The concentration of the X-α-glucoside chromogen was increased to improve the blue-green colouration of Cronobacter strains with weak glucosidase activity. Sodium thiosulphate and ammonium iron (III) citrate are included to differentiate hydrogen sulphide-producing Enterobacteriaceae, which are coloured grey/brown, and sodium deoxycholate is added to inhibit the growth of Gram-positive competitors.

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