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View additional product information for ChargeSwitch™ NoSpin Plasmid Micro Kit - FAQs (CS10201)
6 product FAQs found
We strongly recommend using the Elution Buffer provided in the kit, and do not recommend elution with water. If you need to elute in any other buffer, be sure to use a buffer of pH 8.5-9.0 for efficient DNA elution.
The filter kit is based upon a dual nested column design, which facilitates the fastest and simplest protocol for obtaining high quality plasmid (less than 10 EU/µg DNA). Modified wash buffer 1 removes more endotoxin.
The typical yield is 25 µg from a 5 mL culture for the ChargeSwitch Pro mini kit, 200 µg from a 25 mL culture for the midi kit, and 800 µg from a 100 mL culture from the maxi kit. The plasmid size range for the kits is typically 3-9 kb.
Based on a patented switchable chemistry, ChargeSwitch purification utilizes the charge on a solid surface for nucleic acid capture. At lower pH, the surface chemistry holds a positive charge and attracts nucleic acid. At higher pH, the surface chemistry is neutral and any bound nucleic acid is released.
Sometimes there is insoluble material (probably resin fines) in the precipitated DNA. This material is very easily removed with a 10 second spin. The presence of these particles does not reflect a poor preparation. Once the particles are removed, the DNA performs well in all applications.
We have many products suitable for Miniprep and Midiprep purifications:
The PureLink HiPure Plasmid Miniprep and Midiprep purification kits are based on anion-exchange technology and allow high-yield isolation of highly pure plasmid DNA, equivalent to two passes through CsCl (cesium chloride) gradients. Plasmid isolated with these kits will have the lowest endotoxin levels and will work best for difficult transfections and other challenging applications.
The PureLink HQ Mini Plasmid DNA Purification Kit is a modified silica technology designed to yield high amounts of high-quality plasmid DNA for use in standard mammalian cell transfections, restriction enzyme digestion, PCR, automated sequencing, and transformation. This kit is also available in 96-well format.
The PureLink Quick Plasmid Miniprep Kit is a silica-based purification kit designed to isolate high-quality plasmid DNA suitable for sequencing and cloning applications in 25-45 minutes.
The ChargeSwitch-Pro Plasmid Miniprep Kit uses our ChargeSwitch technology where plasmid is bound to a surface ligand that is positively charged at low pH, and eluted when the pH is raised to 8.5. The ChargeSwitch protocol retains the ease and familiarity of common silica spin-column protocols with binding, washing, and elution steps. Since this method does not require the use of ethanol, chaotropic salts, or organic solvents for nucleic acid purification, the resulting DNA is free of potentially inhibitory compounds.
The ChargeSwitch Plasmid ER and Plasmid Yeast Mini kits use our ChargeSwitch magnetic bead-based technology for isolation of high-quality plasmid DNA.
The ChargeSwitch NoSpin Plasmid Micro kit is a novel magnetic bead technology that eliminates the need for centrifugation in the plasmid purification procedures.
The ChargeSwitch products are easily automated on robotic platforms.