ChargeSwitch® Plasmid Yeast Mini Kit This product, and/or its packaging, incorporates one or more environmental/green features. Click on this icon to learn more.

Catalog number: CS10203

Invitrogen™  Related applications: DNA Extraction

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Sample size 1 ml S. cerevisiae culture; Typical yield up to 25 ng

• Increased transformation efficiency
• Reagents that avoid PCR inhibition
• Purifies DNA with improved downstream performance

The ChargeSwitch® Plasmid Yeast Mini Kit provides an efficient method for purifying high-quality plasmid DNA from 1 ml of Saccharomyces cerevisiae grown in selective minimal media. This kit typically yields up to 25 ng of plasmid DNA. Data generated from transformation and PCR experiments using ChargeSwitch® purified DNA shows improved performance versus DNA purified by other methods (Figure 1). This kit includes all the necessary reagents for the number of preps listed and requires the use of a magnetic accessory. Please see the Accessories Chapter (Chapter 6) to select the best magnetic accessory for your needs.
For Research Use Only. Not for use in diagnostic procedures.


High Throughput Compatibility: Not High Throughput-Compatible (Manual)
Sample Type (General): Yeast
Downstream Application: Cloning, PCR, Sequencing, Southern Blotting, Transformation
Final Product: Plasmid DNA
Isolation Technology: ChargeSwitch® Chemistry
Product Line: ChargeSwitch®
Product Size: 50 preps
Sample Type (Specific): Yeast
Green Features: Fewer resources used, Less hazardous, Less waste
Shipping Condition: Room Temperature

Contents & storage

All components of the ChargeSwitch® Plasmid Yeast Mini Kit are shipped at room temperature. Upon receipt, store components as follows: Mix RNase A in Resuspension Buffer (R4) and store RNase A in Resuspension Buffer (R4) at 4“C. Store Precipitation Buffer (N5) at 4“C to improve performance. Store the remaining kit components at room temperature. All components are guaranteed stable for 6 months when stored properly.


ChargeSwitch® Magnetic Beads (25 mg/ml in 10 mM MES, pH 5.0, 10 mM NaCl, 0.1% Tween 20)
RNase A (5 mg/ml in 10 mM Tris-HCl, pH 8.5, 10 mM EDTA)
ChargeSwitch® Resuspension Buffer (R4)
ChargeSwitch® Lysis Buffer (L9)
ChargeSwitch® Precipitation Buffer (N5)
ChargeSwitch® Wash Buffer (W11)
ChargeSwitch® Wash Buffer (W12)
ChargeSwitch® Elution Buffer (E5; 10 mM Tris-HCl, pH 8.5)


Manuals & protocols