Invitrogen™

ChargeSwitch™ Plasmid Yeast Mini Kit

Green features
Catalog number: CS10203
Invitrogen™

ChargeSwitch™ Plasmid Yeast Mini Kit

Green features
Catalog number: CS10203
Catalog Number
CS10203
Unit Size
50 preps
Price (USD)
218.00
Your Price:
Availability
***
Quantity
Catalog NumberUnit SizePrice (USD)AvailabilityQuantity
CS1020350 preps
218.00
Your Price:
***
Product Overview
Figures
Videos
Documents
FAQ
Citations & References
Additional Information
Recommendations
Recommendations
Sample size 1 ml S. cerevisiae culture; Typical yield up to 25 ng

• Increased transformation efficiency
• Reagents that avoid PCR inhibition
• Purifies DNA with improved downstream performance

The ChargeSwitch™ Plasmid Yeast Mini Kit provides an efficient method for purifying high-quality plasmid DNA from 1 ml of Saccharomyces cerevisiae grown in selective minimal media. This kit typically yields up to 25 ng of plasmid DNA. Data generated from transformation and PCR experiments using ChargeSwitch™ purified DNA shows improved performance versus DNA purified by other methods (Figure 1). This kit includes all the necessary reagents for the number of preps listed and requires the use of a magnetic accessory. Please see the Accessories Chapter (Chapter 6) to select the best magnetic accessory for your needs.

Specifications

Format
Kit
Isolation Technology
ChargeSwitch™ Chemistry
Sample Type
Yeast
Scale
Mini
Final Product Type
Plasmid DNA
For Use With (Application)
PCR, Cloning, Southern Blotting, Sequencing, Transformation
Green Features
Less hazardous, Fewer resources used and less waste
High-throughput Compatibility
Not High-throughput Compatible (Manual)
No. of Reactions
50 Preps
Prep Scale
< 100 µg (Small-Scale) Plasmid DNA
Product Line
ChargeSwitch™
Product Type
Plasmid Yeast Mini Kit
Quantity
50 Preps
Shipping Condition
Room Temperature
Target
Plasmid DNA

Contents & Storage

All components of the ChargeSwitch™ Plasmid Yeast Mini Kit are shipped at room temperature. Upon receipt, store components as follows: Mix RNase A in Resuspension Buffer (R4) and store RNase A in Resuspension Buffer (R4) at 4“C. Store Precipitation Buffer (N5) at 4“C to improve performance. Store the remaining kit components at room temperature. All components are guaranteed stable for 6 months when stored properly.

Components:

ChargeSwitch™ Magnetic Beads (25 mg/ml in 10 mM MES, pH 5.0, 10 mM NaCl, 0.1% Tween 20)
RNase A (5 mg/ml in 10 mM Tris-HCl, pH 8.5, 10 mM EDTA)
ChargeSwitch™ Resuspension Buffer (R4)
ChargeSwitch™ Lysis Buffer (L9)
ChargeSwitch™ Precipitation Buffer (N5)
ChargeSwitch™ Wash Buffer (W11)
ChargeSwitch™ Wash Buffer (W12)
ChargeSwitch™ Elution Buffer (E5; 10 mM Tris-HCl, pH 8.5)

Figures