Invitrogen™

      ChargeSwitch™ PCR Clean-Up Kit

      Catalog number:  CS12000

       Related applications: DNA Extraction

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      Description

      • Sample size 25-50 µl
      • High recovery of DNA fragments
      • Rapid, one tube, 5 minute protocol

      ChargeSwitch® PCR Clean-Up Kit is the fastest and most effective method of removing primers, unincorporated dNTPs, thermostable polymerases and salts (Figure 1). This kit is scalable for use on liquid-handling robots. This kit includes all the necessary reagents for the number of preps listed and requires the use of a magnetic accessory. Please see the Accessories Chapter (Chapter 6) to select the best magnetic accessory for your needs.
      For Research Use Only. Not for use in diagnostic procedures.

      Specifications

      High Throughput Compatibility: High Throughput-Compatible
      Sample Type (General): Enzymatic Reactions (e.g. PCR)
      Downstream Application: Cloning, Microarray Analysis, Next-Generation Sequencing, PCR, Sequencing
      Isolation Technology: ChargeSwitch® Chemistry
      Product Line: ChargeSwitch®
      Product Size: 100 preps
      Sample Type (Specific): PCR Products
      Shipping Condition: Room Temperature

      Contents & storage

      The ChargeSwitch® PCR Clean-Up Kit is shipped at room temperature. Upon receipt, store all components at room temperature. All components are guaranteed stable for 6 months when stored properly.

      Components:

      ChargeSwitch® Magnetic Beads (25 mg/ml in 10 mM MES, pH 5.0, 10 mM NaCl, 0.1% Tween 20)
      ChargeSwitch® Purification Buffer (N5)
      ChargeSwitch® Wash Buffer (W12)
      ChargeSwitch® Elution Buffer (E5; 10 mM Tris-HCl, pH 8.5)

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