Invitrogen™
ChargeSwitch™ PCR Clean-Up Kit
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Description
• Sample size 25-50 µl• High recovery of DNA fragments
• Rapid, one tube, 5 minute protocol
ChargeSwitch® PCR Clean-Up Kit is the fastest and most effective method of removing primers, unincorporated dNTPs, thermostable polymerases and salts (Figure 1). This kit is scalable for use on liquid-handling robots. This kit includes all the necessary reagents for the number of preps listed and requires the use of a magnetic accessory. Please see the Accessories Chapter (Chapter 6) to select the best magnetic accessory for your needs.
For Research Use Only. Not for use in diagnostic procedures.
Specifications
| High Throughput Compatibility: | High Throughput-Compatible |
|---|---|
| Sample Type (General): | Enzymatic Reactions (e.g. PCR) |
| Downstream Application: | Cloning, Microarray Analysis, Next-Generation Sequencing, PCR, Sequencing |
| Isolation Technology: | ChargeSwitch® Chemistry |
| Product Line: | ChargeSwitch® |
| Product Size: | 100 preps |
| Sample Type (Specific): | PCR Products |
| Shipping Condition: | Room Temperature |
Contents & storage
The ChargeSwitch® PCR Clean-Up Kit is shipped at room temperature. Upon receipt, store all components at room temperature. All components are guaranteed stable for 6 months when stored properly.
Components:
ChargeSwitch® Magnetic Beads (25 mg/ml in 10 mM MES, pH 5.0, 10 mM NaCl, 0.1% Tween 20)
ChargeSwitch® Purification Buffer (N5)
ChargeSwitch® Wash Buffer (W12)
ChargeSwitch® Elution Buffer (E5; 10 mM Tris-HCl, pH 8.5)
Components:
ChargeSwitch® Magnetic Beads (25 mg/ml in 10 mM MES, pH 5.0, 10 mM NaCl, 0.1% Tween 20)
ChargeSwitch® Purification Buffer (N5)
ChargeSwitch® Wash Buffer (W12)
ChargeSwitch® Elution Buffer (E5; 10 mM Tris-HCl, pH 8.5)
