• Sample size 25-50 µl • High recovery of DNA fragments • Rapid, one tube, 5 minute protocol
ChargeSwitch® PCR Clean-Up Kit is the fastest and most effective method of removing primers, unincorporated dNTPs, thermostable polymerases and salts (Figure 1). This kit is scalable for use on liquid-handling robots. This kit includes all the necessary reagents for the number of preps listed and requires the use of a magnetic accessory. Please see the Accessories Chapter (Chapter 6) to select the best magnetic accessory for your needs.
For Research Use Only. Not for use in diagnostic procedures.
The ChargeSwitch™ PCR Clean-Up Kit is shipped at room temperature. Upon receipt, store all components at room temperature. All components are guaranteed stable for 6 months when stored properly.
Components:
ChargeSwitch™ Magnetic Beads (25 mg/ml in 10 mM MES, pH 5.0, 10 mM NaCl, 0.1% Tween 20) ChargeSwitch™ Purification Buffer (N5) ChargeSwitch™ Wash Buffer (W12) ChargeSwitch™ Elution Buffer (E5; 10 mM Tris-HCl, pH 8.5)
