GeneCatcher™ gDNA Blood Kit, 3-10 mL

Catalog number: CS21110

Invitrogen™  Related applications: DNA Extraction

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Description

ChargeSwitch® gDNA 3-10 ml Blood Kit uses magnetic bead-based technology to allow rapid and efficient extraction of genomic DNA (gDNA) from human blood, including archived or poorly stored blood samples, without the use of centrifugation.
• Rapid, three-step protocol
• Validated for automation
• Ideal for any downstream application of choice

Cost-Effective, User-Friendly Protocol
ChargeSwitch® gDNA 3-10 ml Blood Kit facilitates purification of up to 300 µg of gDNA from 3 – 10 ml of blood sample. This is done using magnetic bead-based technology that follows a 3-step protocol: DNA Capture, DNA Purification, and Elution (Figure 1). First, cells are lysed, and crude DNA is captured on magnetic beads, leaving most of the cell debris and protein behind in solution. Any residual protein is digested using the Protease and then washed away to leave pure intact DNA. Finally, the pure DNA is then eluted into a small volume, ready for use in any downstream applications (Figure 2). We recommend using the 50 ml Tube Magnetic Separator to obtain the best results.

Flexible Solution for Large-Scale Genotyping and Biobanking Projects
ChargeSwitch® gDNA 3-10 ml Blood Kit has been optimized for scalable and automated gDNA isolation from large blood samples using the Tecan Freedom EVO® liquid handler, providing fully scalable chemistry, high purity, and yield of extracted gDNA. Low elution volumes result in concentrated DNA that is ideal for archiving in standard microcentrifuge tubes.

Improved Performance in Downstream Applications
ChargeSwitch® gDNA 3-10 ml Blood Kit helps ensure reliable performance of the resulting gDNA in a variety of downstream applications, including quantitative PCR, restriction enzyme digestion (Figure 3), and second generation multiplex and single tandem repeat profiling (Figure 4).

For Research Use Only. Not intended for animal or human diagnostic or therapeutic use.
For Research Use Only. Not for use in diagnostic procedures.

Specifications

Sample Type (General): Blood
Isolation Technology: Magnetic Bead
Downstream Application: Forensic Testing, Real-Time Quantitative PCR (qPCR), Sequencing, Southern Blotting, Cloning
High Throughput Compatibility: Not High Throughput-Compatible (Manual)
Product Size: 200 mL
Shipping Condition: Room Temperature

Contents & storage

The GeneCatcher™ gDNA kits include GeneCatcher™ Magnetic Beads, Protease, Protease Buffer, GeneCatcher™ Lysis and Wash Buffers, and GeneCatcher™ Elution Buffer. All components of the GeneCatcher™ gDNA Blood Kits are shipped at room temperature. Upon receipt, store the protease at +4°C and the remaining kit components at room temperature. All components are guaranteed stable for 6 months when properly stored.

Documents

Manuals & protocols