Invitrogen™

Chargeswitch™ Pro Filter Plasmid Mini Kit

Catalog number: CS31103
Invitrogen™

Chargeswitch™ Pro Filter Plasmid Mini Kit

Catalog number: CS31103
Catalog Number
CS31103
Unit Size
100 preps
Price (USD)
514.00
Your Price:
Availability
***
Quantity
Catalog Number
Unit Size
10 preps
Price (USD)
179.00
Your Price:
Availability
***
Quantity
Catalog NumberUnit SizePrice (USD)AvailabilityQuantity
CS31103100 preps
514.00
Your Price:
***
CS3110210 preps
179.00
Your Price:
***
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The Chargeswitch™ -Pro Filter Plasmid Mini Kit includes 10 plasmid minipreps and provides high yield and high purity plasmid with the fastest and the simplest protocol.
The ChargeSwitch™-Pro Plasmid Filter Mini, Midi, Maxi Kits generates high-quality plasmid DNA without the use of harsh or potentially interfering reagents, for better success in downstream applications. The system is based upon a novel dual⁄nested column design which facilitate the fastest and simplest protocol for high quality plasmid. The system is composed of two columns. The inner flat bottomed column, also referred to as “lysate clarification” column, contains filter materials suitable for rapid clarification of bacterial lysate. The outer luer bottomed column, also referred to as “binding” column, contains ChargeSwitch™ (CST) derivatized membrane that immediately binds plasmid DNA from the clarified lysate. This nested-column design enables simultaneous clarification and DNA binding in a single short centrifugation (or vacuum) step. The combined advantages of novel, optimized plastics and a unique chemistry are an extreme competitive advantage of speed and convenience.



To accomplish this, the ChargeSwitch™-Pro Filter Kit provides:

• 100% ethanol-free, guanidinium-free, organics-free, aqueous protocol for a safer, more streamlined method
• Cleaner purified product for improved performance in downstream applications
• A novel Dual⁄nested column design for simultaneous lysate clearing and binding step which offers the fastest and the simplest protocol
• Endotoxin units < 10 EU ⁄ ug DNA for
• Vacuum Elution can be done with the EveryPrep™ Universal Vacuum Manifold (SKU # K211101)



How it works
The ChargeSwitch™ technology replaces the use of ethanol, chaotropic salts, or organic solvents for nucleic acid purification with a simple ion-exchange mechanism. At low pH values (<6.5) the ChargeSwitch™ surface ligand is positively charged and binds plasmid DNA. Raising the pH (>8.5) neutralizes the charge and elutes the bound nucleic acid. The ChargeSwitch™ protocol retains the ease and familiarity of the common silica spin column protocol with a binding, washing, and elution step, making adapting to the ChargeSwitch™ method effortless.

Specifications

Column Type
Spin Column
Format
Spin Column
Isolation Technology
ChargeSwitch™ Chemistry
Sample Type
Bacterial Culture
Scale
Mini
Elution Volume
50 to 150 µl
Final Product Type
Plasmid DNA
For Use With (Application)
Next-Generation Sequencing, Transfection, Cloning, Sequencing, Transformation, Nucleic Acid Labeling, PCR, In Vitro Transcription
High-throughput Compatibility
Not High-throughput Compatible (Manual)
Includes
Miniprep Columns with Collection Tubes, Recovery Tubes, Resuspension Buffer, RNase A, Lysis Buffer, Precipitation Buffer, Wash Buffers 1 and 2, and Elution Buffer
No. of Reactions
100 Preps
Prep Scale
< 100 µg (Small-Scale) Plasmid DNA
Product Line
ChargeSwitch™
Product Type
Pro Filter Plasmid Mini Kit
Quantity
100 Preps
Purity
Transfection Grade
Shipping Condition
Room Temperature
Size
100 preps
Starting Material Volume
5 ml
Target
Plasmid DNA
System Type
ChargeSwitch™
Test Time
15 min
Yield
30 µg

Contents & Storage

The ChargeSwitch™-Pro Plasmid Miniprep Kit contains Miniprep Columns with Collection Tubes, Recovery Tubes, Resuspension Buffer, RNase A, Lysis Buffer, Precipitation Buffer, Wash Buffers 1 and 2, and Elution Buffer. Store at room temperature. Guaranteed stable for 6 months when properly stored.

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