Invitrogen™

Chargeswitch™ Pro Filter Plasmid Midi Kit

Catalog number: CS31104
Invitrogen™

Chargeswitch™ Pro Filter Plasmid Midi Kit

Catalog number: CS31104
Catalog Number
CS31104
Unit Size
25 preps
Price (USD)
600.00
Your Price:
Availability
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Quantity
Catalog NumberUnit SizePrice (USD)AvailabilityQuantity
CS3110425 preps
600.00
Your Price:
***
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The Chargeswitch™ -Pro Filter Plasmid Midi Kit includes 25 plasmid midipreps and provides high yield and high purity plasmid with the fastest and the simplest protocol.
The ChargeSwitch™-Pro Plasmid Filter Mini, Midi, Maxi Kits generates high-quality plasmid DNA without the use of harsh or potentially interfering reagents, for better success in downstream applications. The system is based upon a novel dual⁄nested column design which facilitate the fastest and simplest protocol for high quality plasmid. The system is composed of two columns. The inner flat bottomed column, also referred to as “lysate clarification” column, contains filter materials suitable for rapid clarification of bacterial lysate. The outer luer bottomed column, also referred to as “binding” column, contains ChargeSwitch™ (CST) derivatized membrane that immediately binds plasmid DNA from the clarified lysate. This nested-column design enables simultaneous clarification and DNA binding in a single short centrifugation (or vacuum) step. The combined advantages of novel, optimized plastics and a unique chemistry are an extreme competitive advantage of speed and convenience.



To accomplish this, the ChargeSwitch™-Pro Kit provides:

• 100% ethanol-free, guanidinium-free, organics-free, aqueous protocol for a safer, more streamlined method
• Cleaner purified product for improved performance in downstream applications
• A novel Dual⁄nested column design for simultaneous lysate clearing and binding step which offers the fastest and the simplest protocol
• Endotoxin units < 10 EU ⁄ ug DNA for
• Vacuum Elution can be done with the EveryPrep™ Universal Vacuum Manifold (SKU # K211101)



How it works
The ChargeSwitch™ technology replaces the use of ethanol, chaotropic salts, or organic solvents for nucleic acid purification with a simple ion-exchange mechanism. At low pH values (<6.5) the ChargeSwitch™ surface ligand is positively charged and binds plasmid DNA. Raising the pH (>8.5) neutralizes the charge and elutes the bound nucleic acid. The ChargeSwitch™ protocol retains the ease and familiarity of the common silica spin column protocol with a binding, washing, and elution step, making adapting to the ChargeSwitch™ method effortless.
For Research Use Only. Not for use in diagnostic procedures.

Specifications

Column Type
Spin Column
Format
Spin Column
Isolation Technology
ChargeSwitch™ Chemistry
Sample Type
Bacterial Culture
Scale
Midi
Elution Volume
0.5 to 1 mL
Final Product Type
Plasmid DNA
For Use With (Application)
Next-Generation Sequencing, Transfection, Cloning, Sequencing, Transformation, Nucleic Acid Labeling, PCR, In Vitro Transcription
High-throughput Compatibility
Not High-throughput Compatible (Manual)
Includes
Columns with Resuspension Buffer, RNase A, Lysis Buffer, Precipitation Buffer, Wash Buffers 1 and 2, and Elution Buffer
Prep Scale
100-200 µg (Medium-Scale) Plasmid DNA
Product Line
ChargeSwitch™
Product Type
Pro Filter Plasmid Midi Kit
Quantity
25 Preps
Purity
Transfection Grade
Shipping Condition
Room Temperature
Shelf Life
Guaranteed stable for 6 months when properly stored.
Starting Material Volume
25 ml
Target
Plasmid DNA
System Type
ChargeSwitch™
Test Time
30 min
Yield
200 μg

Contents & Storage

The ChargeSwitch™-Pro Plasmid Kit contains Columns with Resuspension Buffer, RNase A, Lysis Buffer, Precipitation Buffer, Wash Buffers 1 and 2, and Elution Buffer. Store at room temperature. Guaranteed stable for 6 months when properly stored.

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