Invitrogen™

ChargeSwitch™-Pro PCR Clean-Up Kit

Green features
Catalog number: CS32250
Invitrogen™

ChargeSwitch™-Pro PCR Clean-Up Kit

Green features
Catalog number: CS32250
Catalog Number
CS32250
Unit Size
250 preps
Price (USD)
638.00
Your Price:
Availability
***
Quantity
Catalog Number
Unit Size
10 preps
Price (USD)
34.90
Your Price:
Availability
***
Quantity
Catalog Number
Unit Size
50 preps
Price (USD)
210.00
Your Price:
Availability
***
Quantity
Catalog NumberUnit SizePrice (USD)AvailabilityQuantity
CS32250250 preps
638.00
Your Price:
***
CS3201010 preps
34.90
Your Price:
***
CS3205050 preps
210.00
Your Price:
***
Product Overview
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Additional Information
Recommendations
Recommendations
The ChargeSwitch™-Pro PCR Clean-up Kit purifies high-quality DNA fragments without the use of ethanol or harsh salts known to interfere with enzymatic reactions. The ChargeSwitch™-Pro Kit provides:
• 100% ethanol-free, guanidinium-free, organic solvent-free protocols
• Improved downstream performance
• Complete kit for fast, hassle-free setup
• 20% fewer handling steps than most PCR clean-up protocols
• Purification of up to 500 µl of PCR product in one column


How It Works

ChargeSwitch™ technology employs a completely novel mechanism that replaces harsh reagents such as chaotropic salts, ethanol, or organic solvents and isopropanol precipitation with a simple ion-exchange procedure. In this method, DNA binds to a surface ligand that is positively charged at low pH, and is eluted when the pH is raised to 8.5 (Figure 1). The ChargeSwitch™-Pro protocol retains the ease and familiarity of common silica spin-column protocols with binding, washing, and elution steps (Figure 2).


Better Downstream Results

Since ChargeSwitch™ technology does not require the use of ethanol, chaotropic salts, or organic solvents for nucleic acid purification, the resulting DNA is free of enzymatic inhibitors and thus shows better downstream performance (Figure 3). These compounds are known to inhibit many enzymatic reactions such as PCR, cloning, restriction enzyme digestion, and array CGH labeling. Expect to see reduced variability in your sequencing applications, with reads in excess of 800 bp with Phred scores >20 (Figure 4); clean restriction enzyme digestions (Figure 5); and removal of dNTPs and primers in excess of 98% (Figure 6).

Specifications

Format
Kit
Isolation Technology
ChargeSwitch™ Chemistry
Sample Type
PCR Product
For Use With (Application)
Next-Generation Sequencing, Cloning, Sequencing, PCR
Green Features
Fewer resources used and less waste, Less hazardous
High-throughput Compatibility
Not High-throughput Compatible (Manual)
No. of Reactions
250 Preps
Product Line
ChargeSwitch™
Product Type
PCR Clean-Up Kit
Quantity
250 Preps
Shipping Condition
Room Temperature
Target
DNA from Enzymatic Reactions (e.g. PCR)

Contents & Storage

The ChargeSwitch™-Pro PCR Clean-up Kit contains columns, collection tubes, recovery tubes, binding buffer, wash buffer, and elution buffer.

Store at room temperature. Guaranteed stable for 6 months when properly stored.

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