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IL12A and IL12B Recombinant Human Protein, His Tag

Catalog number: CT011H08H5

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Description

Interleukin 12 (Interleukin 12A And Interleukin 12B Heterodimer) (IL12A and IL12B), recombinant human protein is supplied as a lyophilized powder. It is suitable for use in protein studies such as protein structure analysis and protein-protein interactions. In general, recombinant proteins can also be used as an immunogen, as a protein standard, or in cell biology research applications.

This recombinant heterodimer protein was expressed from a DNA sequence encoding the p35 subunit of human IL-12, termed as IL12A (NP_000873.2) (Met 1-Ser 219) fused to a polyhistidine tag at the C-terminus, constructed the plasmid 1, and from a DNA sequence encoding the p40 subunit of human IL-12, termed as IL12B (NP_002178.2) (Met 1-Ser 328) fused to a polyhistidine tag at the C-terminus, constructed the plasmid 2. The two plasmids were co-expressed and the heterodimer was purified.

Activity: Measured by its ability to bind biotinylated recombinant human IL12RB1 in a functional ELISA.

Formulation: Lyophilized in 140 mM NaCl, 2.7 mM KCl, 10 mM Na2HPO4, 1.8 mM KH2PO4, pH7.4, 5% Mannitol, 5% Trehalose, 0.02% Tween80.

Reconstitution: Dissolve the protein in sterile double distilled water to a concentration of 0.2 mg/ml or lower. It is recommended that the protein be aliquoted and be used as soon as possible. Store aliquots under sterile conditions at -20°C. Avoid repeated freeze-thaw cycles.

Expiration Date: Expires one year from date of receipt when stored as instructed.

This protein is manufactured by Sino Biological Inc.

For Research Use Only. Not for use in diagnostic procedures.

Specifications

Research Category: Immunology, Inflammation, Microbiology
Endotoxin Level: < 1 EU/µg
Expression: Mammalian
Form: Lyophilized
Gene Aliases: Interleukin-12
Gene Symbol: IL12A and IL12B
Molecular Weight: 60.2 kDa
Product Line: Sino Biological®
Product Size: 5 µg
Protein Family: Cytokines & Receptors
Protein Name: interleukin 12 (interleukin 12A and interleukin 12B heterodimer)
Protein Subtype: Interleukins
Protein Tag or Fusion: His Tag
Purity: ≥ 90% by SDS-PAGE
Species: Human
Validated Application: Assay Standard

Contents & storage

Includes 1 vial of lyophilized protein. Store at -20 °C.

Documents

Manuals & protocols

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